Table 1

PCR primers used in this study

Primer* Sequence (5′→3′)
[-21M13]TGTAAAACGACGGCCAGT
DHCR-58–38 [-21M13]-GGTTCAAGAAGGAAAAGTTCCC
DHCR684-703 [-21M13]-TCGGGAAGTGGTTTGACTTC
DHCR930-949 [-21M13]-CTGTGTCTGGCTGCCTTATC
DHCR949-967 [-21M13]-CTTTACACGCTGCAGGGTC
DHCR-ins21-39 [-21M13]-CGTGTGTCAGAGGCAGAGC
[M13rev]CAGGAAACAGCTATGACC
DHCR979-960 [M13rev]-ACACCAAGTACAGACCCTGC
DHCR996-978 [M13rev]-CAGCTGCACGGGGTGGTAC
DHCR1563-1544 [M13rev]-GGGCTCTCTCCAGTTTACAG
DHCR-ins113-95 [M13rev]-GTCAAGCGGTGCTTTGCCC
  • PCR conditions are available on request. *Numbering of nucleotide residues according to previously published 7-DHCR cDNA sequence.6