Primer* | Sequence (5′→3′) |
[-21M13] | TGTAAAACGACGGCCAGT |
DHCR-58–38 | [-21M13]-GGTTCAAGAAGGAAAAGTTCCC |
DHCR684-703 | [-21M13]-TCGGGAAGTGGTTTGACTTC |
DHCR930-949 | [-21M13]-CTGTGTCTGGCTGCCTTATC |
DHCR949-967 | [-21M13]-CTTTACACGCTGCAGGGTC |
DHCR-ins21-39 | [-21M13]-CGTGTGTCAGAGGCAGAGC |
[M13rev] | CAGGAAACAGCTATGACC |
DHCR979-960 | [M13rev]-ACACCAAGTACAGACCCTGC |
DHCR996-978 | [M13rev]-CAGCTGCACGGGGTGGTAC |
DHCR1563-1544 | [M13rev]-GGGCTCTCTCCAGTTTACAG |
DHCR-ins113-95 | [M13rev]-GTCAAGCGGTGCTTTGCCC |
PCR conditions are available on request. *Numbering of nucleotide residues according to previously published 7-DHCR cDNA sequence.6