Intron | Forward primer (5′→3′) | Reverse primer (5′→3′) | Size | Temp (°C) | Cycles |
Intron 6 | M13F1-150-atggacttcagccgatttc | M13R1-150-agtcattgccctttttcc | ∼5 kb | 59 | 42 |
Intron 7 | M13F-cttaaagacagatgaacgg | M13R-tggagcaagaagaagaag | ∼4 kb | 60 | 40 |
Intron 8 | M13F-aacttatttccccgctac | M13R-gaggaacacgaacacaaa | 1.4 kb | 57 | 35 |
Intron 9 | M13F-tacctctgggtcagcttt | M13R-gggatagtgaaaacatgg | 1.6 kb | 58 | 23 |
↵1-150 M13F: cgccagggttttcccagtcacgac
M13R: tttcacacaggaaacagctatgac