Data on the PAX4 fragments analysed in this study and PCR conditions
| Exon size (bp) | Primer (5′>3′) | Cosmid AC000359 (exon positions) | mRNAAF043978 | Ta (°C) | MgCl2 (mM) | |||||
| 5′UTR | atacctctgtctcacgccca | 31609-31362 | 1–206 | 63.5 | 1,5 | |||||
| ggcttcctgtccccatcactg | ||||||||||
| 1 (120) | atacctctgtctcacgccca | 31074-30881 | 207–326 | 63.5 | 1,5 | |||||
| ggcttcctgtccccatcactg | (31042-30923) | |||||||||
| 2 (216) | catgcctcacctctccctg | 30653-30363 | 327–542 | 63.5 | 1,5 | |||||
| tcttttccagccccagtgtg | (30617-30402) | |||||||||
| 3 (76) | atctctccactcaacaccct | 30110-29927 | 543–618 | 58 | 1,5 | |||||
| acgagaaagggctttgagaa | (30079-30004) | |||||||||
| 4 (126) | atctgaccagaggaatcacc | 29460-29231 | 619–744 | 61 | 1,5 | |||||
| tgtcacactgaggactctct | (29043-29278) | |||||||||
| 5 (83) | ttggggttgtagcaggtggg | 29148-28929 | 745–827 | 60 | 1,5 | |||||
| cttcctccttgtggtttgta | (29053-28971) | |||||||||
| 6 (70) | gatgtcaggcccaaggaagggtcaa | 28730-28501 | 828–897 | 67 | 1,5 | |||||
| gatgactgagcggcagatggatg | (28612-28543) | |||||||||
| 7 (56) | caaccttcctgcctctcacc | 27610-27420 | 898–953 | 65 | 1,5 | |||||
| cccatgagcccttcagtctt | (27529-27474) | |||||||||
| 8 (142) | ctggaagctaaggttcttct | 27243-27011 | 954–1095 | 61 | 1,5 | |||||
| tcagtcgaccctccctacat | (27205-27064) | |||||||||
| 9 (143) | aagctctctctatttgtccc | 27660-26407 | 1096–1238 | 65 | 1,5 | |||||
| cacacaggagggagcatca | (26735-26593) | |||||||||
| 3′UTR1 | cggcccactactgtatggct | 26760-26407 | 1239–2020 | 59 | 1,5 | |||||
| agagtgggcataggggtgctcata | ||||||||||
| 3′UTR2 | tcgatgcctctctggcgcat | 26451-26050 | 55 | 3 | ||||||
| aatgccatgatataatatca | ||||||||||
| 3′UTR3 | tgtatgtatgtgtgcatgcg | 26122-25773 | 53 | 3 | ||||||
| cccctggagcatgtataaat | ||||||||||
Ta, annealing temperature; MgCl2, final concentration.