Exon | Forward primer | Reverse primer | Product size (bp) | Annealing temp (°C) |
1a | GCCCCCGCCGTAGCAGT | ACGAAGAGGAGCCCCAGGTG | 318 | 621-150 |
1b | TACTATTTCTCCTACCCGCTGTTT | CGACCCCACCGCCTTCT | 321 | 571-150 |
1c | CGGCGGCGGCAGTGAGAG | ATGAGGGCGCGGGAGAAG | 284 | 621-150 |
2 | TTTTTATGTATTACCTCTCAAC | TGGGATGGCTATAAACAAAT | 204 | 55 |
3 | TTAGTTGGGAAATGTAGAT | TATGTTAAAAAGCCTGGAC | 246 | 55 |
4 | TTTTTACCTTCTCTGTTG | AAGCTTTATTATTTTATGTTAGTT | 156 | 55 |
5 | GTTCAGCTACAATTTTCTAATCAC | TATGATCAACTTAAGCAGGAAT | 321 | 55 |
6 | ATGTTAGGTTGTATTTTCA | CAAGGTATTTATTATCTATTTCAC | 273 | 55 |
7 | TCATAGGGCTTAGGCTTCA | ATGGATTCAGTAACAGATGGTATT | 224 | 55 |
8 | CTGTTTGGGAAGATGCT | CTCAAGGACAAGATAAAGTT | 261 | 60 |
9 | TGGCCTCATAGCTTACATTTTTAG | ATACGACAATATTGGAAACAGAG | 220 | 55 |
10 | GTGCTAGATTTTCAACATA | GCCCTTCTTTAAAACTTCTTCC | 270 | 55 |
11 | GAATTTAGTAGGACCCACT | GCCACATTAAAAATATCATA | 230 | 55 |
12 | ATGGCCAAGGTTAAAAATACAA | CTGGAAGAAAATAGTGAAT | 281 | 55 |
13 | CTTTTCCTGTCATTTGCTGTTT | GATGGTAGTTCTTGTTTCTGCTCT | 173 | 55 |
14 | ATCATTAATTCTGAAATTAG | ATAAACCAAATCCAAA | 154 | 48 |
15 | AAAAAGCGGGAGGGGAAATA | TGGGCAACAGAGTGAGACC | 248 | 561-150 |
16 | TGTATGTATTTTTAAGTGCCTGAC | TTACAATATAGAAGACAAAGAAA | 125 | 55 |
17 | AACAGCAGCATCATTACTTT | GTTCTGCAGGTTTACAA | 241 | 55 |
↵1-150 Requires the addition of 2 mol/l Betaine (Sigma) to the PCR reaction.