Primer
|
Sequence (5′-3′)
|
Tanneal (°C) long PCR/nested PCR
|
494-130kP1 | gaggaaatacggatttgggg | 58 |
SY83F | cttgaatcaaagaaggccct | 58/60 |
494-146kP2 | atggcttcatcccaactgag | 58 |
ELT2 | cctgctaacagtataatgaatggg | 58/60 |
12f2E | gttcttgagctgggaagatctcag | 58/65 |
12f2J | gtgctttccaagcatttggag | 65 |
12f2L | ttaattcagccctctgagcg | 58 |
12f2M | tatggtctctatggaagtagg | 58 |
12f2N | aaagcctgtggtcagcctgag | 58 |
12f2D | ctgactgatcaaaatgcttacagatc | See Materials and methods |
12f2F | tcttctagaatttcttcacagaattg | See Materials and methods |
3′Sry15 | cttgattttctgctagaacaag | See Materials and methods |
3′Sry16 | tgtcgttacataaatgggcac | See Materials and methods |