Primers and annealing temperatures used in the amplification of exons from the human T gene. F indicates the forward (sense) and R the reverse (antisense) primers. *indicates the 5′ UTR of exon 1 and † exon 1 coding sequence
| Primer | Sequence (5′ → 3′) | Annealing temp | Size of PCR product |
|---|---|---|---|
| TX1Fb* | CCAGAGACCTACACTAGTAGAGCC | 58°C | 228 bp (exon 1) |
| TX1Rb* | AAGCCCAGACTCGCTACCTGAG | ||
| TX1F † | ATCTCAGGTAGCGAGTCTGGGCTTC | 61°C | 269 bp (exon 1) |
| TX1R † | ACCCACCTGCCGTTCTTGGTCAC | ||
| TX2F | TGCGCCCGCCCCGTGTTTTCCAG | 68°C | 309 bp (exon 2) |
| TX2R | TGGCGGGCTCCTCACACCTACCT | ||
| TX3F | TGCCACCAATCCTGTATCTGTCTC | 63°C | 191 bp (exon 3) |
| TX3R | AAGCGATCCGCCTCTGTCCTTCTCA | ||
| TX4F | GGTGCCAAATTAAATCCCCTG | 53°C | 173 bp (exon 4) |
| TX4R | CTGCATACTTCTTTGCCAAGG | ||
| TX5F | CCTTGGCAAAGAAGTATGCAG | 53°C | 192 bp (exon 5) |
| TX5R | TGACAGAGTGCAACAAACCG | ||
| TX6F | CTCAGCACTGTCTTTCTCACAG | 58°C | 223 bp (exon 6) |
| TX6R | GGCAGAATGAGGCTGAGGACTC | ||
| TX7F | TGTTCTTGGAAGACACCATC | 53°C | 221 bp (exon 7) |
| TX7R | TCCCAGGATGCTTTGCACCA | ||
| TX8F | CCCTGCTCACTGGTGTCTTTC | 56°C | 338 bp (exon 8) |
| TX8R | AGTCACTGCATCTTTCGGGAC | ||
| TX8Fb | AGGCAGCAGCCGTGTCCAACG | 57°C | 256 bp (exon 8, 3′ end) |
| TX8Rb | ATGGGTGAGGGGTGTGTAGTG | 58.5°C | 159 bp (exon 8, 5′ end) |