Region | Primer sequence | Annealing site | Annealing temp (°C) |
Regulatory | F 5′-TAGGCTCAGTGGGAGAAAGG-3′ | 196–215 | 65 |
R 5′-CAGTTGGCAGGTGTGTCG-3′ | 1359–1342 | 65 | |
Exon 1 | F 5′-GCCGCCCTCCTGCTGCTTCT | 1298–1318 | 57 |
R 5′-CCAATGCTAAAACTTGTTGAGACC | 2528–2505 | 57 | |
Intron | F 5′-AGGAGGTTGTGTCTTGTAGCC-3′ | 2127–2147 | 65 |
R 5′-AGTGCCTGTGTAGGGGAAGC-3′ | 3896–3877 | 65 | |
Exon 2 | F 5′-TCCCCACTTAACCACTTTGC-3′ | 3559–3578 | 63 |
R 5′-ACTTCATAGCTGACCATCTTCC-3′ | 4547–4526 | 63 |