The identification of Y chromosome translocations following Distamycin A treatment

Clin Genet. 1981 May;19(5):335-42. doi: 10.1111/j.1399-0004.1981.tb00720.x.

Abstract

Pretreatment of PHA-stimulated lymphocyte cultures from normal males with 100 micrograms/ml Distamycin A for the final 24 h of incubation led to a striking decondensation of the heterochromatic portion of the long arm of the Y chromosome. The despiralized region of Yq maintained its fluorescent properties in this altered morphologic state. Two cases of Y chromosome translocation (Y/22 and Y/X) were identified with this technique. A review of the literature revealed 62 similar cases in which the phenotypic-karyotypic correlations were instrumental in the prenatal counseling of the family with the Y/22 translocation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Banding
  • Chromosomes, Human, 21-22 and Y*
  • Distamycins*
  • Female
  • Humans
  • Male
  • Pregnancy
  • Prenatal Diagnosis
  • Pyrroles*
  • Sex Chromosomes*
  • Translocation, Genetic*
  • Y Chromosome*

Substances

  • Distamycins
  • Pyrroles