Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Leuk Res. 2009 Sep;33(9):1276-81. doi: 10.1016/j.leukres.2008.10.010. Epub 2008 Nov 21.

Abstract

We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to approximately 1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Antigens, Neoplasm / genetics*
  • Chromosome Deletion*
  • Chromosomes, Human, Pair 22*
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
  • Nucleic Acid Hybridization*
  • Polymerase Chain Reaction

Substances

  • Antigens, Neoplasm
  • PRAME protein, human