Abstract
A novel human nucleotide sugar transporter (NST) which transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) has been identified, cloned and characterized. The strategy for the identification of the novel NST involved a search of the expressed sequence tags database for genes related to the human UDP-galactose transporter-related isozyme 1, followed by heterologous expression of a candidate gene (hUGTrel7) in Saccharomyces cerevisiae and biochemical analyses. Significantly more UDP-GlcA and UDP-GalNAc were translocated from the reaction medium into the lumen of microsomes prepared from the hUGTrel7-expressing yeast cells than into the control microsomes from cells not expressing hUGTrel7. The possibility that this transporter participates in glucuronidation and/or chondroitin sulfate biosynthesis is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Bacterial Toxins / pharmacology
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Base Sequence
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Biological Transport / drug effects
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CHO Cells
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Cricetinae
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Gene Expression
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Hemolysin Proteins / pharmacology
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Humans
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Microsomes / metabolism
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Molecular Sequence Data
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Monosaccharide Transport Proteins / genetics*
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Monosaccharide Transport Proteins / metabolism
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Substrate Specificity / physiology
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Transformation, Genetic
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Transport Vesicles / metabolism
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Uridine Diphosphate Glucuronic Acid / metabolism*
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Uridine Diphosphate Glucuronic Acid / pharmacokinetics
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Uridine Diphosphate N-Acetylgalactosamine / metabolism*
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Uridine Diphosphate N-Acetylgalactosamine / pharmacokinetics
Substances
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Bacterial Toxins
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DNA, Complementary
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Hemolysin Proteins
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Monosaccharide Transport Proteins
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SLC35D1 protein, human
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staphylococcal alpha-toxin
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Uridine Diphosphate Glucuronic Acid
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Uridine Diphosphate N-Acetylgalactosamine