Molecular characterization of human UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity

FEBS Lett. 2001 Apr 20;495(1-2):87-93. doi: 10.1016/s0014-5793(01)02358-4.

Abstract

A novel human nucleotide sugar transporter (NST) which transports both UDP-glucuronic acid (UDP-GlcA) and UDP-N-acetylgalactosamine (UDP-GalNAc) has been identified, cloned and characterized. The strategy for the identification of the novel NST involved a search of the expressed sequence tags database for genes related to the human UDP-galactose transporter-related isozyme 1, followed by heterologous expression of a candidate gene (hUGTrel7) in Saccharomyces cerevisiae and biochemical analyses. Significantly more UDP-GlcA and UDP-GalNAc were translocated from the reaction medium into the lumen of microsomes prepared from the hUGTrel7-expressing yeast cells than into the control microsomes from cells not expressing hUGTrel7. The possibility that this transporter participates in glucuronidation and/or chondroitin sulfate biosynthesis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Toxins / pharmacology
  • Base Sequence
  • Biological Transport / drug effects
  • CHO Cells
  • Cricetinae
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Expression
  • Hemolysin Proteins / pharmacology
  • Humans
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins / genetics*
  • Monosaccharide Transport Proteins / metabolism
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Substrate Specificity / physiology
  • Transformation, Genetic
  • Transport Vesicles / metabolism
  • Uridine Diphosphate Glucuronic Acid / metabolism*
  • Uridine Diphosphate Glucuronic Acid / pharmacokinetics
  • Uridine Diphosphate N-Acetylgalactosamine / metabolism*
  • Uridine Diphosphate N-Acetylgalactosamine / pharmacokinetics

Substances

  • Bacterial Toxins
  • DNA, Complementary
  • Hemolysin Proteins
  • Monosaccharide Transport Proteins
  • SLC35D1 protein, human
  • staphylococcal alpha-toxin
  • Uridine Diphosphate Glucuronic Acid
  • Uridine Diphosphate N-Acetylgalactosamine

Associated data

  • GENBANK/AB044343