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Flicker image comparison of 2-D gel images for putative protein identification using the 2DWG meta-database

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Abstract

With the availability of two-dimensional (2-D) gel electrophoresis databases that have many characterized proteins, it may be possible to compare a researcher’s gel images with those in relevant databases. This may lead to the putative identification of unknown protein spots in a researcher’s gel with those characterized in a given database, saving the researcher time and money by suggesting monoclonal antibodies to try in confirming these identifications. We have developed two tools to help with this comparison: (1) Flicker, http://www.lecb.ncifcrf.gov/flicker/, a Java applet program running in the researcher’s Web browser, to visually compare their gels against gels on the Internet; and (2) the 2DWG meta-database, http://www.lecb.ncifcrf.gov/2dwgDB/, a searchable database of locations of 2-D electrophoretic gel images found on the Internet. Recent additions to Flicker allow users to click on a protein spot in a gel that is linked to a federated 2D gel database, such as SWISS-2DPAGE, and have it retrieve a report from that Web database for that protein.

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Authors and Affiliations

  1. IPS/LECB NCI/FCRDC, 21702, Frederick, MD

    Peter F. Lemkin

  2. SAIC/FCRDC, 21702, Frederick, MD

    Gregory Thornwall

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  1. Peter F. Lemkin
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  2. Gregory Thornwall
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Correspondence to Peter F. Lemkin.

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Lemkin, P.F., Thornwall, G. Flicker image comparison of 2-D gel images for putative protein identification using the 2DWG meta-database. Mol Biotechnol 12, 159–172 (1999). https://doi.org/10.1385/MB:12:2:159

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