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Embryonic lethal mutation in mice induced by retrovirus insertion into the α1(I) collagen gene

Nature volume 304, pages 315–320 (1983)Cite this article

Abstract

Experimental insertion of a retrovirus into the germ line of mice has resulted in an embryonic recessive lethal mutation. Integration of the proviral genome occurred at the 5′ end of the α1(I) collagen gene, leading to a complete transcriptional block. Developmental arrest of embryos homozygous at the mutated allele coincides with high expression of the gene in normal embryogenesis. Insertion mutagenesis by retroviruses may offer a general approach to the identification and isolation of genes which are transcriptionally active during mammalian development.

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Authors and Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, 2000, Hamburg, 20, FRG

    Angelika Schnieke, Klaus Harbers & Rudolf Jaenisch

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  1. Angelika Schnieke
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  2. Klaus Harbers
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  3. Rudolf Jaenisch
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Schnieke, A., Harbers, K. & Jaenisch, R. Embryonic lethal mutation in mice induced by retrovirus insertion into the α1(I) collagen gene. Nature 304, 315–320 (1983). https://doi.org/10.1038/304315a0

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