Abstract
Soluble kappa-elastin peptides were shown to stimulate the expression of MMP-2 (but not MMP-9) byhuman fibrosarcoma HT-1080 cells, both at the protein and mRNA levels; maximal effect being observedat a concentration of 25 mg/ml of kappa-elastin. The stimulatory effect could be reproduced using Val-Gly-Val-Ala-Pro-Gly (VGVAPG) peptide, an elastin-derived hydrophobic hexapeptide which represented theelastin receptor binding sequence of tropoelastin. Furthermore, treatment of cells with lactose (30 mM),which dissociated 67-kDa elastin binding protein (EBP) from cell surfaces, completely abolished this effect,suggesting that the elastin receptor could mediate such a response. Using a specific monoclonal antibody,67-kDa EBP was detected in HT-1080 membrane preparations by Western immunoblotting. Following treat-mentwith 25 mg/ml kappa-elastin or 200 mg/ml VGVAPG, increased levels of the active 62-kDa form ofMMP-2 were found in HT-1080 cell extracts. Stimulation of MT1-MMP mRNA expression by treatmentwith elastin-derived peptides (EDPs) was shown by competitive polymerase chain reaction (PCR). A reversezymography analysis revealed that EDPs also stimulated TIMP-2 (but not TIMP-1) production by HT-1080cells. Competitive PCR confirmed increased TIMP-2 mRNA expression by such treatment. These resultssuggest that occupancy of the 67-kDa elastin receptor by elastin-derived peptides enhanced both expressionand activation of proMMP-2 and consequently, could promote the invasive/metastatic ability of tumor cellsexpressing this receptor. ©Kluwer Academic Publishers
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References
Starkey JR, 1990, Cell-matrix interactions during tumor invasion. Cancer Metastasis Rev, 9, 113-23.
Jacob MP and Hornebeck W, 1985, Isolation and characterization of insoluble and kappa-elastins. Front Matrix Biol, 10, 92-129.
Damiano VV, Tsang A, Christner P, et al.1979, Immunologic localization of elastin by electron microscopy. Am J Pathol, 96, 439-48.
Castronovo V, 1993, Laminin receptors and laminin-binding proteins during tumor invasion and metastasis. Invasion Metastasis, 13, 1-30.
Timar J, Lapis K, Fulop T, et al. 1991, Interaction between elastin and tumor cell lines with different metastatic potential; in vitroand in vivostudies. J Cancer Res Clin Oncol, 117, 232-8.
Mecham RP, Hinek A, Entwistle R et al. 1989, Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein. Biochemistry, 28, 3716-22.
Hinek A, 1994, Nature and the multiple functions of the 67-kD elastin-/laminin binding protein. Cell Adhes Commun, 2, 185-93.
Jacob MP, Fulop T, Foris G and Robert L, 1987, Effect of elastin peptides on ion fluxes in mononuclear cells, fibroblasts, and smooth muscle cells. Proc Natl Acad Sci USA, 84, 995-9.
Landeau JM, Pellat B and Hornebeck W, 1994, Increased secretion of latent elastase activity following contact between human skin fibroblasts and elastin derived peptides. Cell Biol Int, 18, 111-17.
Kamoun A, Landeau JM, Godeau G, et al. 1995, Growth stimulation of human skin fibroblasts by elastin-derived peptides. Cell Adhes Commun, 3, 273-81.
Svitkina T and Parsons DF, 1993, Binding of some metastatic tumor cell lines to fibrous elastin and elastin peptides. Int J Cancer, 53, 824-8.
Yu AE, Hewitt RE, Kleiner DE and Stetler-Stevenson WG, 1996, Molecular regulation of cellular invasion. Role of gelatinase A and TIMP-2. Biochem Cell Biol, 74, 823-31.
Woessner JF, 1991, Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J, 5, 2145-54.
Liotta LA, Tryggvason K, Garbisa S, et al.1981, Partial purification and characterization of a neutral protease which cleaves type IV collagen. Biochemistry, 20, 100-4.
Senior RM, Griffin GL, Fliszar CJ et al. 1991, Human 92-and 72-kilodalton type IV collagenases are elastases. J Biol Chem, 266, 7870-5.
Yusa T, Blood CH and Zetter BR, 1989, Tumor cell interactions with elastin: implications for pulmonary metastasis. Am Rev Respir Dis, 140, 1458-62.
Parsons DF, 1995, Some tumor cell protein kinases activated by receptors as markers, including elastin receptors. Cancer Invest, 13, 629-36.
Iwamoto Y, Nomizu M, Yamada Y et al. 1996, Inhibition of angiogenesis, tumour growth and experimental metastasis of human fibrosarcoma cells HT1080 by a multimeric form of the laminin sequence Tyr-Ile-Gly-Ser-Arg (YIGSR). Br J Cancer, 73, 589-95.
Brown PD, Levy AT, Margulies IMK, et al.1990, Independent expression and cellular processing of Mr 72,000 type IV collagenase and interstitial collagenase in human tumorigenic cell lines. Cancer Res, 50, 6184-91.
Okada Y, Gonoji Y, Naka K, et al. 1992, Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. Purification and activation of the precursor and enzymic properties. J Biol Chem, 267, 21712-19.
Okada A, Bellocq JP, Rouyer N, et al. 1995, Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas. Proc Natl Acad Sci USA, 92, 2730-4.
Stetler-Stevenson WG, Brown PD, Onisto M, Levy AT and Liotta LA, 1990, Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues. J Biol Chem, 265, 13933-8.
Strongin AY, Collier I, Bannikov G, et al. 1995, Mechanism of cell surface activation of 72-kDa type IV collagenase. Isolation of the activated form of the membrane metalloprotease. J Biol Chem, 270, 5331-8.
Kueng W, Silber E and Eppenberger U, 1989, Quantification of cells cultured on 96-well plates. Anal Biochem, 182, 16-19.
Mazzieri R, Masiero L, Zanetta L, et al. 1997, Control of type IV collagenase activity by components of the urokinase-plasmin system: a regulatory mechanism with cell-bound reactants. EMBO J, 16, 2319-32.
Noël AC, Polette M, Lewalle JM, et al. 1994, Coordinate enhancement of gelatinase A mRNA and activity levels in human fibroblasts in response to breast adenocarcinoma cells. Int J Cancer, 56, 331-6.
Mecham RP, Hinek A, Cleary EG, et al.1988, Development of immunoreagents to ciliary zonules that react with protein components of elastic fibers. Biochem Biophys Res Commun, 151, 822-6.
Emonard H, Takiya C, Dreze S, et al.1989, Interstitial collagenase (MMP-1), gelatinase (MMP-2) and stromelysin (MMP-3) released by human fibroblasts cultured on acellular sarcoid granulomas (Sarcoid Matrix Complex, SMC). Matrix, 9, 382-8.
Beranger JY, Godeau G, Frances C, et al.1994, Presence of gelatinase A and metalloelastase type protease at the plasma membrane of human skin fibroblasts. Influence of cytokines and growth factors on cell-associated metalloendopeptidase levels. Cell Biol Int, 18, 715-22.
Emonard H and Hornebeck W, 1997, Binding of 92 kDa and 72 kDa progelatinases to insoluble elastin modulates their proteolytic activation. Biol Chem, 378, 265-71.
Chomczynski P and Sacchi N, 1987, Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Biochem, 162, 156-9.
Riedy MC, Timm EA and Stewart CC, 1995, Quantitative RT-PCR for measuring gene expression. Biotechniques, 18, 70-6.
Collier IE, Wilhelm SM, Eisen AZ, et al. 1988, H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement collagen. J Biol Chem, 263, 6579-87.
Takino T, Sato H, Yamamoto E and Seiki M, 1995, Cloning of a human gene potentially encoding a novel matrix metalloproteinase having a C-terminal transmembrane domain. Gene 155, 293-8.
Hammani K, Blakis A, Morsette D, et al. 1996, Structure and characterization of the human tissue inhibitor of metalloproteinases-2 gene. J Biol Chem, 271, 25498-505.
Tokunaga K, Nakamura Y, Sakata K, et al.1987, Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers. Cancer Res, 47, 5616-19.
Hinek A, Wrenn DS, Mecham RP and Barondes SH, 1988, The elastin receptor: a galactoside-binding protein. Science, 239, 1539-41.
Sato H, Takino T, Okada Y, et al. 1994, A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature, 370, 61-5.
Hinek A, Rabinovitch M, Keeley F, et al.1993, The 67-kD elastin/laminin-binding protein is related to an enzymatically inactive, alternatively spliced form of ?-galactosidase. J Clin Invest, 91, 1198-205.
Hinek A, 1996, Biological role of the non-integrin elastin/laminin receptor. Biol Chem, 377, 471-80.
Kopitz J, von Reitzenstein C, Muhl C and Canz M, 1994, Role of plasma membrane ganglioside sialidase of human neuroblastoma cells in growth control and differentiation. Biochem Biophys Res Commun, 199, 1188-93.
Kunitomo M and Jay M, 1985, Elastin fragmentinduced monocyte chemotaxis. The role of desmosine. Inflammation, 9, 183-8.
Fulop T, Jacob MP, Varga ZS, et al.1986, Effect of elastin peptides on human monocytes: Ca2+ mobilization, stimulation of respiratory burst and enzyme secretion. Biochem Biophys Res Commun, 141, 92-8.
Mecham RP, Hinek A, Griffin GL, et al.1989, The elastin receptor shows structural and functional similarities to the 67-kDa tumor cell laminin receptor. J Biol Chem, 264, 16652-7.
Mecham RP, Whitehouse L, Hay M, et al.1991, Visualization of elastin/laminin receptor complexes with gold-tagged ligands: modulation by carbohydrate and cytoskeleton attachment. J Cell Biol, 113, 187-94.
D'Errico A, Garbisa S, Liotta LA, et al.1991, Augmentation of type IV collagenase, laminin receptor, and Ki67 proliferation antigen associated with human colon, gastric, and breast carcinoma progression. Mod Pathol, 4, 239-46.
Nomura H, Sato H, Seiki M, et al.995, Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. Cancer Res, 55, 3263-6.
Yoshizaki T, Sato H, Maruyama Y, et al. 1997, Increased expression of membrane type 1-matrix metalloproteinase in head and neck carcinoma. Cancer, 79, 139-44.
Tsunezuka Y, Kinoh H, Takino T, et al. 1996, Expression of membrane-type matrix metalloproteinase 1 (MT1-MMP) in tumor cells enhances pulmonary metastasis in experimental metastasis assay. Cancer Res, 56, 5678-83.
Emmert-Buck MR, Emonard HP, Corcoran ML et al. 1995, Cell surface binding of TIMP-2 and pro-MMP-2/TIMP-2 complex. FEBS Lett, 364, 28-32.
Corcoran ML, Hewitt RE, Kleiner DE and Stetler-Stevenson WG, 1996, MMP-2: expression, activation and inhibition. Enzyme Prot, 49, 7-19.
Visscher DW, Hoyhtya M, Ottosen SK, et al. 1994, Enhanced expression of tissue inhibitor of metalloproteinase-2 (TIMP-2) in the stroma of breast carcinomas correlates with tumor recurrence. Int J Cancer, 59339-44.
Grignon DJ, Sakr W, Toth M, et al. 1994, High levels of tissue inhibitor of metalloproteinase-2 (TIMP-2) expression are associated with poor outcome in invasive bladder cancer. Cancer Res, 56, 1654-9.
Varga Z, Jacob MP, Robert L and Fulop T, 1989, Identification and signal transduction mechanism of elastin peptide receptor in human leukocytes. FEBS Lett, 258, 5-8.
Timar J, Diczhazi CS, Ladanyi A, et al. 1995, Interaction of tumour cells with elastin and the metastatic phenotype. In: Chadwick DS and Goode JA, eds. The Molecular Biology and Pathology of Elastic Tissues. Chichester: John Wiley and Sons (Ciba Foundation Symposium 192), pp. 321-37.
Blood CH, Sasse J, Brodt P and Zetter BR, 1988, Identification of a tumor-cell receptor for VGVAPG, an elastin-derived chemotactic peptide. J Cell Biol, 107, 1987-95.
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Brassart, B., Randoux, A., Hornebeck, W. et al. Regulation of matrix metalloproteinase-2(gelatinase A, MMP-2), membrane-type matrixmetalloproteinase-1 (MT1-MMP) and tissue inhibitorof metalloproteinases-2 (TIMP-2) expression byelastin-derived peptides in human HT-1080 fibrosarcoma cell line. Clin Exp Metastasis 16, 489–500 (1998). https://doi.org/10.1023/A:1006550503612
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DOI: https://doi.org/10.1023/A:1006550503612