Genome screening by comparative genomic hybridization

doi:10.1016/S0168-9525(97)01244-4

Trends in Genetics

Volume 13, Issue 10, October 1997, Pages 405-409

https://doi.org/10.1016/S0168-9525(97)01244-4 Get rights and content

Abstract

Comparative genomic hybridization (CGH) provides a molecular cytogenetic approach for genome-wide scanning of differences in DNA sequence copy number. The technique is now attracting wide-spread interest, especially among cancer researchers. The rapidly expanding database of CGH publications already covers about 1500 tumors and is beginning to reveal genetic abnormalities that are characteristic of certain tumor types or stages of tumor progression. Six novel gene amplifications, as well as a locus for a cancer-predisposition syndrome, have been discovered based on CGH data. CGH has now been established as a first-line screening technique for cancer researchers and will serve as a basis for ongoing efforts to develop high-resolution next-generation genome scanning, such as the microarray technology.

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Citation Excerpt :
The development of array CGH (a-CGH) by combining DNA microarray with CGH helped in improving the sensitivity of the test. Convectional array technique had 5–10 Mb resolution while array CGH had a resolution of 40 kb [40]. Array CGH method used single cells and in 2008 first healthy baby was born after array CGH [41–43].
Application of Preimplantation Genetic Testing technique for the diagnosis of monogenic disorder in the embryos of couples who were at risk of transferring genetic disorder to their child began in the mid 1980’s. In the mid-1990s, this method was used as a method for embryo selection in patients undergoing in vitro fertilization, by analyzing maximum number of chromosomes possible, with anticipation of selecting chromosomally normal embryo which may lead to higher implantation and decrease pregnancy loss. We discuss here the evolution of Preimplantation Genetic Testing method, the challenges faced during this process of development, to evaluate its efficiency and drawbacks of different methods and to summarize the recent progress of the new methods and its potential application in increasing the live birth rate. All relevant articles addressing Preimplantation Genetic Testing, Preimplantation Genetic Screening or Diagnosis published from 1980’s to 2019’s were reviewed and analyzed. Preimplantation Genetic Testing has helped any couples in having a healthy baby and though biopsy technique is considered invasive due to the removal of blastomere from the embryo, blastocyst biopsy is considered as the most reliable among the biopsy methods available. Non-invasive preimplantation genetic testing are considered to replace conventional biopsy technique but their limitations despite their advantage needs to be addressed and require validation for clinical application.
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Trends in Genetics

ReviewGenome screening by comparative genomic hybridization

Abstract

Chromosome Res.

Blood

Blood

Blood

Science

Hum. Genet.

Am. J. Hum. Genet.

Cytogenet. Cell Genet.

Prenat. Diag.

Prenat. Diag.

Hum. Mol. Genet.

Am. J. Pathol.

Cancer Res.

Genes Chromosomes Cancer

Genes Chromosomes Cancer

Cancer Res.

Blood

Genes Chromosomes Cancer

Review
Genome screening by comparative genomic hybridization