Abstract
αB-crystallin (α-BC), a member of the small heat-shock proteins (sHSP), is constitutively expressed in the vertebrate lens and in non-ocular tissues including the central nervous system (CNS). In this study we investigated the expression of α-BC in the rat brain after middle cerebral artery occlusion (MCAO). α-BC transcript and protein were transiently expressed 4 h after MCAO/reperfusion in the pyramidal neurons in the peri-infarct region of the ischemic hemisphere. Beginning 2 days after MCAO, significant α-BC induction appeared in reactive astrocytes in the penumbra, and this induction was sustained for several days. In addition, levels of MAPKAPK-2, one of the α-BC upstream kinases, and its phosphorylated form were upregulated gradually and peaked 4 days after ischemia/reperfusion injury. The immunohistochemical study indicated that α-BC was co-localized with MAPKAPK-2 and p-MAPKAPK-2. Furthermore, p38β MAPK, an upstream kinase of MAPKAPK-2, which has been known to be involved in compensatory responses to stress, was also co-localized with α-BC in the penumbra. Our results suggest that the p38β-dependent α-BC induction in neurons and astrocytes in the penumbra may play an important role in the postischemic brain.
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Abbreviations
- α-BC:
-
αB-Crystalline
- sHSP:
-
Small heat-shock protein
- CNS:
-
Central nervous system
- MCAO:
-
Middle cerebral artery occlusion
- GFAP:
-
Glial fibrillary acidic protein
- BBB:
-
Blood–brain barrier
- MAPKAPK-2:
-
Mitogen-activated protein kinase-activated protein kinase-2
- LEDGF:
-
Lens epithelium-derived growth factor
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This work was supported by a grant from the Health Planning Technology and Evaluation Board (02-PJ1-PG3–21304–0009) for J-K Lee.
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Piao, CS., Kim, SW., Kim, JB. et al. Co-induction of αB-crystallin and MAPKAPK-2 in astrocytes in the penumbra after transient focal cerebral ischemia. Exp Brain Res 163, 421–429 (2005). https://doi.org/10.1007/s00221-004-2197-2
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DOI: https://doi.org/10.1007/s00221-004-2197-2