Skip to main content
SpringerLink
Log in

Prevention of chromosomal breakage in Fanconi's anemia by cocultivation with normal cells

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

The frequency of aberration in cultured lymphocytes from patients with Fanconi's anemia was significantly reduced when the cells were cocultivated with normal human lymphocytes. The results suggest that most of the chromosomal aberrations observed in cultured cells from Fanconi patients arise during cultivation and that the presence of normal cells prevents chromosomal damage by means of a hitherto unknown mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bloom GE, Warner S, Gerald PS, Diamond LK (1966) Chromosome abnormalities in constitutional aplastic anemia. N Engl J Med 274:8–14

    PubMed  Google Scholar 

  • Brown WT, Epstein J, Little JB (1976) Progeria cells are stimulated to repair DNA by cocultivation with normal cells. Exp Cell Res 97:291–296

    PubMed  Google Scholar 

  • Dutrillaux B, Couturier J, Viegas-Péquignet E, Schaison G (1977) Localization of chromatid breaks in Fanconi's anemia, using three consecutive stains. Hum Genet 37:65–71

    PubMed  Google Scholar 

  • Germain D, Requin Ch (1970) Anomalies chromosomiques dans les cytopénies constitutionelles. Nouv Rev Fr Hematol 10: 107–117

    PubMed  Google Scholar 

  • Hayashi K, Schmid W (1975) The rate of sister chromatid exchanges parallel to spontaneous chromosome breaikage in Fanconi's anemia and to Trenimon-induced aberrations in human lymphocytes and fibroblasts. Hum Genet 29:201–206

    Google Scholar 

  • Hungerford DA (1965) Leucocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KC. Stain Technol 40: 333–337

    PubMed  Google Scholar 

  • Joenje H, Eriksson AW, Frants RR, Arwert F, Houwen B (1978) Erythrocyte superoxide dismutase deficiency in Fanconi's anemia. Lancet I:204

    Article  Google Scholar 

  • Koskull H von, Aula P (1973) Nonrandom distribution of chromosome breaks in Fanconi's anemia. Cytogenet Cell Genet 12:423–424

    PubMed  Google Scholar 

  • Nordenson I (1977) Effect of superoxide dismutase and catalase on spontaneously occurring chromosome breaks in patients with Fanconi's anemia. Hereditas 86:147–150

    PubMed  Google Scholar 

  • Polani PE (1976) Cytogenetics in Fanconi anaemia and related chromosome disorders. Congenital disorders of erythropoiesis. Ciba Found Symp 37:261–302

    Google Scholar 

  • Sasaki MS (1975) Is Fanconi's anaemia defective in a process essential to the repair of DNA cross links? Nature 257: 501–503

    PubMed  Google Scholar 

  • Schroeder TM, Drings P (1973) Verlaufsbeobachtung einer Fanconi-Anämie bei einem Erwachsenen. Verh Dtsch Ges Inn Med 29:477–481

    Google Scholar 

  • Sperling K, Wegner R-D, Riehm H, Obe G (1975) Frequency and distribution of sister chromatid exchanges in a case of Fanconi's anemia. Hum Genet 27:227–230

    Article  Google Scholar 

  • Varela MA, Sternberg WH (1967) Preanaemic state in Fanconi's anaemia. Lancet II:566–567

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Genetics, University of Umeå, S-90185, Umeå, Sweden

    I. Nordenson

  2. Department of Pediatrics, University of Umeå, S-90185, Umeå, Sweden

    B. Björksten

  3. Department of Internal Medicine, University of Lund, Sweden

    B. Lundh

Authors
  1. I. Nordenson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Björksten
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Lundh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nordenson, I., Björksten, B. & Lundh, B. Prevention of chromosomal breakage in Fanconi's anemia by cocultivation with normal cells. Hum Genet 56, 169–171 (1980). https://doi.org/10.1007/BF00295689

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00295689

Keywords

Search

Navigation