Summary
Three families are reported showing transmission of a previously described variant, which is not associated with any clinical abnormality. The variant involves additional material at the band 9p12, which shows homogeneous staining of intermediate density with GTL- and RBG-banding, and negative staining with CBG-banding. The region stains positively with Feulgen stain. In situ hybridization with total genomic human DNA, cloned alpha satellite, satellite III, and ribosomal DNA all show no hybridization to the 9p12 variant. Two members of one of the families show the largest 9p12 variant yet reported; two other carriers in this family have inherited a variant of decreased size. It is suggested that the 9p12 variants are homogeneously staining regions. Using the ISCN three-letter convention, this variant could be described as hsr(9)(p12).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Archidiacono N, Pecile V, Rocchi M, Dalpra L, Nocera G, Simoni G (1984) A rare non-heterochromatic 9p+ variant in two amniotic cell cultures. Prenat Diagn 4:231–233
Barker PE, Lau YF, Hsu TC (1980) A heterochromatic homogeneously staining region (HSR) in the karyotype of a human breast carcinoma cell line. Cancer Genet Cytogenet 1:311–319
Biedler JL, Spengler BA (1976) Metaphase chromosome anomaly: association with drug resistance and cell specific products. Science 191:185–187
Boldyreff B, Winking H, Weith A, Traut W (1988) Evidence for in situ amplification of a germ line homogeneously staining region in the mouse. Cytogenet Cell Genet 47:84–85
Buckle VJ, Craig IW (1986) In situ hybridization. In: Davies KE (ed) Human genetic diseases: a practical approach. IRL Press, Oxford, pp 85–100
Buckton KE, O'Riordan ML, Ratcliffe S, Slight J, Mitchell M, McBeath S, Keay AJ, Barr D, Short M (1980) A G-band study of chromosomes in liveborn infants. Ann Hum Genet 43:277–279
Choo KH, Brown R, Webb GC, Craig I, Filby RG (1987) Genomic organisation of human centromeric alpha satellite DNA: characterisation of a human 17 alpha satellite sequence DNA. J Mol Biol 6:297–305
Choo KH, Vissel B, Brown R, Filby RG, Earle E (1988) Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14 and 21: implications for recombination between nonhomologous and Robertsonian translocations. Nucleic Acids Res 16:1273–1284
Cowell JK (1982) Double minutes and homogeneously staining regions: gene amplification in mammalian cells. Annu Rev Genet 16:21–59
Djalali M, Barbi G, Steinbach P (1986) An unusual variant chromosome 9 due to disturbance of normal chromatin condensation at band p12? Clin Genet 30:80
Gilbert F, Balaban G, Brangman D, Herrmann N, Lister A (1983) Homogeneously staining regions and tumorigenicity. Int J Cancer 31:765–768
John B, King M (1983) Population cytogenetics of Atractomorpha similis. I. C-band variation. Chromosoma 88:57–68
Miller OJ, Tantravahi R, Miller DA, Yu L-C, Szabo P, Prensky W (1979) Marked increase in ribosomal RNA gene multiplicity in a rat hepatoma cell line. Chromosoma 71:183–195
Pathak SN (1978) Natural and induced polymorphism of constitutive heterochromatin in mammals. Nucleus 21:12–21
Roberts JM, Buck LB, Axel R (1983) A structure for amplified DNA. Cell 33:53–63
Schimke R (ed) (1982) Gene amplification. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Spedicato FS, Di Comite A, Tohidast-Akrad M (1985) An unusual variant chromosome 9 with an extra C-negative. G-dark segment in the short arm. Clin Genet 28:162–165
Sutherland GR, Eyre H (1981) Two unusual G-band variants of the short arm of chromosome 9. Clin Genet 19:331–334
Thompson PW, Roberts SH (1987) A new variant of chromosome 16. Hum Genet 76:100–101
Traut W, Winking H, Adolf S (1984) An extra segment in chromosome 1 of wild mus musculus: a C-band positive homogeneously staining region. Cytogenet Cell Genet 38:290–297
Webb GC, Neuhaus P (1979) Chromosome organization in the Australian plague locust Chortoicetes terminifera. II. Banding variants of the B-chromosome. Chromosoma 70:205–238
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Webb, G.C., Krumins, E.J.M., Eichenbaum, S.Z. et al. Non C-banding variants in some normal families might be homogeneously staining regions. Hum Genet 82, 59–62 (1989). https://doi.org/10.1007/BF00288273
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00288273