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Comprehensive spectrum of BRCA1 and BRCA2 deleterious mutations in breast cancer in Asian countries
  1. Ava Kwong1,2,3,5,
  2. Vivian Y Shin1,
  3. John C W Ho1,4,
  4. Eunyoung Kang6,
  5. Seigo Nakamura7,
  6. Soo-Hwang Teo8,9,
  7. Ann S G Lee10,11,12,
  8. Jen-Hwei Sng13,
  9. Ophira M Ginsburg14,
  10. Allison W Kurian3,
  11. Jeffrey N Weitzel15,
  12. Man-Ting Siu1,
  13. Fian B F Law2,4,
  14. Tsun-Leung Chan2,4,
  15. Steven A Narod14,
  16. James M Ford3,
  17. Edmond S K Ma2,4,
  18. Sung-Won Kim6
  1. 1Department of Surgery, The University of Hong Kong, Hong Kong, Hong Kong
  2. 2Hong Kong Hereditary Breast Cancer Family Registry, Hong Kong, Hong Kong
  3. 3Departments of Medicine (Oncology) and Genetics, Stanford University School of Medicine, Stanford, California, USA
  4. 4Department of Molecular Pathology, Hong Kong Sanatorium & Hospital, Hong Kong, Hong Kong
  5. 5Department of Surgery, Hong Kong Sanatorium & Hospital, Hong Kong, Hong Kong
  6. 6Department of Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea
  7. 7Department of Surgery, Division of Breast Surgical Oncology, Showa University School of Medicine, Tokyo, Japan
  8. 8Cancer Research Initiatives Foundation, Sime Darby Medical Centre, Subang Jaya, Selangor, Malaysia
  9. 9Faculty of Medicine, University Malaya Cancer Research Institute, University Malaya, Subang Jaya, Malaysia
  10. 10Division of Medical Sciences, National Cancer Centre, Singapore, Singapore
  11. 11Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
  12. 12Office of Clinical & Academic Faculty Affairs, Duke-NUS Graduate Medical School, Singapore, Singapore
  13. 13Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
  14. 14Women's College Research Institute, University of Toronto, Toronto, Ontario, Canada
  15. 15Division of Clinical Cancer Genetics, City of Hope National Medical Center, Duarte, California, USA
  1. Correspondence to Dr Ava Kwong, Breast Surgery Division, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong; akwong{at}asiabreastregistry.com

Abstract

Approximately 5%–10% of breast cancers are due to genetic predisposition caused by germline mutations; the most commonly tested genes are BRCA1 and BRCA2 mutations. Some mutations are unique to one family and others are recurrent; the spectrum of BRCA1/BRCA2 mutations varies depending on the geographical origins, populations or ethnic groups. In this review, we compiled data from 11 participating Asian countries (Bangladesh, Mainland China, Hong Kong SAR, Indonesia, Japan, Korea, Malaysia, Philippines, Singapore, Thailand and Vietnam), and from ethnic Asians residing in Canada and the USA. We have additionally conducted a literature review to include other Asian countries mainly in Central and Western Asia. We present the current pathogenic mutation spectrum of BRCA1/BRCA2 genes in patients with breast cancer in various Asian populations. Understanding BRCA1/BRCA2 mutations in Asians will help provide better risk assessment and clinical management of breast cancer.

  • Cancer: breast
  • BRCA1
  • BRCA2
  • Asians
  • Germline mutations

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Introduction

Breast cancer is the most frequent malignancy and the most leading cause of cancer deaths in women worldwide. In 2012, there were estimated to be 522 000 breast cancer deaths, which accounted for 14.7% of all cancer deaths among women (GLOBOCAN 2012, http://globocan.iarc.fr/Pages/fact_sheets_population.aspx). According to the American Cancer Society, the 5-year survival rate for patients with breast cancer ranged from 22% to 100% according to the cancer stages, but this varies for different geographical origins.1 Familial breast cancer accounts for 5%–10% of all breast cancers and is known to be caused by germline mutations in certain genes.2 Deleterious mutations in breast cancer-associated genes (BRCA1 and BRCA2) account for 20%–40% of the familial breast cancer.3 ,4 Women with BRCA1/BRCA2 mutations have very high lifetime risks of developing breast and ovarian cancer.5 Meta-analyses indicated that BRCA1 mutation carriers have a 57%–65% lifetime probability of developing breast cancer while BRCA2 carriers have a 45%–49% lifetime probability.6 ,7 Although there are increasing reports from Asia, the majority of studies to date have focused on the prevalence and spectrum of BRCA1 and BRCA2 mutations in white populations from Europe and North America, African and African–American populations. Asians comprise 60% of the 7 billion people in the world and this population is rapidly increasing. The two most populated countries alone, China and India, constitute 37% of the world population. According to the US Census Bureau, 4.8% of the American populations are Asians. Hence, there is a need for better understanding of the mutation spectrum of these high-penetrance genes and cancer risk prediction in Asians, so that appropriate genetic testing and management/surveillance programmes can be implemented. Globally, there are important differences in age-specific incidence rates of breast cancer between countries and between ethnic groups.8 In this review, we summarise the current spectrum of deleterious BRCA1 and BRCA2 mutations including novel, previously unpublished mutations among Asian countries, and where available, those Asians residing in Western countries.

Study population

Our cohort represents a study population from 47 Asian countries under the geographical definition from GLOBOCAN, together with regions including Hong Kong and Taiwan, and the Asian populations residing in North America. Mutational information of BRCA1 and BRCA2 genes (GenBank accession no.: U14680.1 and U43746.1 respectively) were collected from the Hong Kong Hereditary Breast Cancer Family Registry (http://www.asiabreastregistry.com), Korean Hereditary Breast Cancer study, study groups of the Asian Hereditary Breast Cancer Consortium (ABRCA) and collaborating centres in North America (Canada and USA). We also consolidated data from the Breast Cancer Information Core (BIC), Human Genome Variation Society, unpublished data and published literatures of BRCA1/BRCA2 mutations in Asia (including India, Pakistan, Turkey, Iran, Iraq, Syria, Yemen and other Asian countries). Only pathogenic mutations in BRCA1/BRCA2 genes, which cause deleterious effects to the protein functions, were included in this report. Other genetic variants of uncertain clinical significance (namely VUS) are out of the scope of this study and will be published as a separate study by the ABRCA Consortium group.

This study was approved by the Institutional Review Board (IRB) of the University of Hong Kong and IRBs of the collaborating centres. Mutational information of BRCA1 and BRCA2 were generated, specifically (1) mutation type; (2) date/year of test result; (3) patient ethnicity; (4) country where the mutation was identified; (5) frequency of entries in BIC; and (6) whether the mutation has been reported to be recurrent or founder mutation. Literature search also included mutation frequencies in specific groups including patients with breast cancer at young age, triple-negative breast cancer (TNBC) and bilateral breast cancer. The distribution of BRCA1 and BRCA2 mutations in each country was also recorded.

Selection criteria

The inclusion criteria for BRCA1/BRCA2 genetic testing varied among research groups in our study and in the published literatures, and are summarised in table 1. In general, the study samples consisted of high-risk individuals of familial breast and/or ovarian cancer who satisfied any one of the following criteria:

  1. having a strong family history of breast cancer and/or ovarian cancer;

  2. having early-onset breast cancer, diagnosed at age less than 50 years;

  3. having bilateral breast cancer;

  4. having TNBC;

  5. having male breast cancer;

  6. having medullary type pathology;

  7. having a family history of cancer, other than breast or ovary, which are known to be related to BRCA1/BRCA2 mutations, such as stomach and prostate;

  8. having ovarian cancer and a family history of breast cancer.

Table 1

Inclusion criteria for BRCA1/BRCA2 genetic testing by research groups in different countries (any one of the risk factors)

BRCA1/BRCA2 pathogenic mutations in Asians

In this review, all the novel or unpublished BRCA1/BRCA2 mutation information were collected through the ABRCA including research groups from Bangladesh, Mainland China, Hong Kong, Indonesia, Japan, Korea, Malaysia, Philippines, Singapore, Thailand and Vietnam, together with the collaborating centres in North America. Published mutation data were obtained through literature search. To the best of our knowledge, this is the most up-to-date overview of the BRCA1/BRCA2 pathogenic mutation spectrum in Asian population available. Table 2 illustrates the total numbers of BRCA1/BRCA2 distinct mutations and mutation-positive cases, and a selection of the most frequent mutations identified in each Asian country and in North America. The full spectra of germline BRCA1/BRCA2 deleterious mutations are listed in the online supplementary tables S1 and S2. To date, 510 distinct types of deleterious BRCA1/BRCA2 mutations (268 BRCA1 and 242 BRCA2) have been identified in Asian patients with breast cancer, most of which are frameshift or nonsense mutations.

Table 2

Overview of Asian BRCA1/BRCA2 pathogenic mutations included in this review

The most common BRCA1 mutation reported in our data set was 185delAG (c.68_69delAG; no. of cases: 29), which is a well-known Ashkenazi Jewish mutation, and this mutation was detected in the Indian and Arabic populations, but not in Eastern Asia. The second most reported BRCA1 mutation was c.390C>A (no. of cases: 28), which was solely found in the Japanese and Korean patients. Other common BRCA1 mutations include c.470_471delCT (no. of cases: 16; BIC entries: 10) and c.981_982delAT (no. of cases: 9; BIC entries: 9). BRCA1 c.470_471delCT mutation was identified in Chinese patients that were populated in Hong Kong, Malaysia and the USA, and was also identified in Japanese and Pakistani patients. BRCA1 c.981_982delAT was seen in Chinese and Korean patients in Hong Kong, Korea, Malaysia and Shanghai. Both of these mutations were identified as recurrent mutation in Chinese population, which contributed to 20.6% of all BRCA1 mutations in the Chinese cohort in Hong Kong, Southern China.14

The most common BRCA2 mutations were BRCA2 c.7480C>T (no. of cases: 53; BIC entries: 11), c.1399A>T (no. of cases: 29; BIC entries: 2) and c.3744_3747delTGAG (no. of cases: 26; BIC entries: 8). They were frequently observed in Korean and Chinese patients.

To date, there are 28 distinct mutations in BRCA1 gene and 41 mutations in BRCA2 gene that have neither been previously reported nor been listed in the BIC database (see online supplementary tables S1 and S2); thus they are considered to be novel mutations identified in Asian populations. Among these novel mutations, four BRCA1 and eight BRCA2 distinct mutations had been identified in more than one individual. In this study, 40 BRCA1 and 25 BRCA2 mutations, comprising 12.7% (65 of 510) of all distinct mutations, were listed in the BIC database with records of Asian ethnicities only, suggesting that these could be the Asian-specific pathogenic mutations.

There were 41 distinct BRCA1 mutations and 35 BRCA2 mutations reported in multiple locations across Asia, and some were also found in the Asian populations of North America (see online supplementary tables S1 and S2). These recurrent mutations accounted for 37.5% (233 of 622) of all BRCA1 mutation-positive cases and 36.9% (215 of 583) of all BRCA2 mutation-positive cases in this report (table 2). In total, the frequency of BRCA1 mutations outnumber that of BRCA2 mutations (622 vs 583). However, this was not the case in all Asian countries; in China, Hong Kong, Korea and Philippines, BRCA2 mutations outnumber that of BRCA1 mutations (table 2). In most non-Asian countries the total frequency of BRCA2 mutations observed in Asian populations is more than that for BRCA1 mutations. However, the reverse situation was usually observed in Caucasian or other non-Asian populations.61–63 A number of common mutations specific to non-European populations have been reported in Hispanic, African–American, Middle Eastern and Asian populations.64 ,65

In Asia, the prevalence of BRCA1/BRCA2 mutations from unselected patients with breast cancer had been reported to range from 0.8% to 4.4% (all age groups).34 ,66 ,67 This is comparable to data from Western countries (1.8% to 3.6%).68 Nonetheless, the reported prevalence varies from country to country; a recent report indicated that the prevalence of BRCA1/BRCA2 mutations in Korea from patients with non-familial high-risk breast cancer was 9.8%.69

There are mutations which have been recurrently seen across different countries. To the best of our knowledge, there is no report showing that families sharing the same mutation among the countries were related. All the haplotyping was done in their own countries as it is rather difficult to obtain the samples for analysis. This would be an important piece of missing information that needs to be answered; therefore a consortium has been established to clarify this issue.

BRCA1/BRCA2 mutations, in particular, BRCA1 mutations are associated with young age of onset of cancer and with TNBC. Table 3 summarises the relationship of BRCA1/BRCA2 mutations and these phenotypic characteristics which have been reported in Asia. The definition of young age for the purposes of testing varied between studies, and cut-offs ranged from 30 to 45 years. In Singapore, young age was defined as <30 years, whereas in Hong Kong young age was defined as <45 years, the suggested age of testing criteria under the National Comprehensive Cancer Network Clinical Practice Guidelines in Oncology (NCCN Guidelines) for ‘Genetic/Familial High-Risk Assessment: Breast and Ovarian’. Based on positive family history, studies from Korea, Hong Kong and Malaysia contained probands with the highest proportions of patients (ranged from 50% to 61%) who had family history of breast and/or ovarian cancer. However, this proportion varied greatly among different Asian countries, due to their relatively small study cohort sizes, and further study is needed in order to provide more accurate estimation in different countries.

Table 3

Comparison of BRCA1 and BRCA2 studies among breast cancer cases for family history in Asian countries

TNBC is featured by the absence or lack of oestrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 phenotype, and it accounts for 10%–20% of all breast cancers. TNBC has been reported to be associated with BRCA1/BRCA2 mutation, where 50% of high-risk patients with TNBC and a positive family history were tested positive for BRCA1/BRCA2 mutation.80 BRCA1/BRCA2 mutation was detected in approximately 40% of unselected Ashkenazi Jewish women with TNBC, and the majority of these were BRCA1 mutation.81 In the USA, there was a higher proportion of BRCA1 mutation (20%) than BRCA2 mutation (4%) in patients with TNBC.82 Likewise, in the Asian populations, more TNBC cases were found to be associated with BRCA1 mutation than BRCA2 mutations. Several Chinese cohort studies showed that the prevalence of BRCA1 mutations among selected patients with TNBC ranged from 18.6% to 36.8%, while BRCA2 mutation only ranged from 7.3% to 10.5%.13 ,75 ,76 A similar discrepancy in BRCA1/BRCA2 mutation prevalence (24.5% vs 3.6%) has also been reported in a Malaysian study of patients with TNBC with Malay, Chinese and Indian ancestries.77 Taken together, these observations suggested that BRCA1 mutation dominancy in patients with TNBC was present in both Asian and the West.

Published data had shown that the prevalence of BRCA1/BRCA2 mutation was identified in approximately 20% of Korean patients with bilateral breast cancers.26 ,69 A similar mutation analysis revealed that 20% of patients with bilateral breast cancer were BRCA1 mutation carriers and 12% were BRCA2 mutation carriers; 8% of the patients had a family history of breast and ovarian cancer in a first-degree or second-degree relative.83 Similarly, a higher frequency of BRCA1 mutation was also seen in women with bilateral breast cancer in Canada.84

In addition to the widely studied BRCA1 and BRCA2, genetic testing for mutations in other familial breast cancer-associated genes, for instance, PTEN, TP53, ATM, CHEK2 and PALB2, using multiple-gene sequencing panels had shown its important clinical values with the advances in next-generation sequencing technology. In a recent study, 141 women tested negative for BRCA1/BRCA2 mutations had been identified for 16 pathogenic variants in other cancer susceptibility genes, giving a prevalence of 11.4% using multiple-gene sequencing.85 However, the interpretation of results from such multiple-gene panels and their applications in routine diagnostic utility remain to be optimised. Moreover, the mutation data on cancer susceptibility genes other than BRCA1/BRCA2 in Asian populations is also largely unknown.

This review encapsulates the up-to-date BRCA1/BRCA2 mutation spectrum in Asia and Asians residing in Western countries and provides new insights into the distribution and characteristics of BRCA1 and BRCA2 mutations in Asia. The identification of common mutations in some ethnic groups or geographical locations raises the possibility of defining more efficient strategies for genetic testing. In particular, the high frequency of these mutations may provide information for genetic test panels that facilitate the ease of genetic testing for these individuals in Asian countries and those Asians who reside in the West, and may have value in optimising efficient strategies for genetic testing for BRCA1 and BRCA2 mutations associated with breast and ovarian cancer susceptibility which would be particularly useful. The discrepancies of the frequencies of mutations vary across countries or even within the same country, maybe due to different selection criteria, genetic testing methods, different availability and cost of testing. In Japan, a ‘Myriad Genetics’-based laboratory is used, while the other Asian countries did the testing in their own laboratories, most of which were supported by research grants and donations or were even self-financed. Hence, the limitations to access to these tests would be the cost and affordability of the test. The National Comprehensive Cancer Network Guidelines for ‘Genetic/Familial High-Risk Assessment: Breast and Ovarian’ provide a good backbone but it may need to be adjusted to improve applicability due to the lower incidence in breast cancer in Asia, lack of family history and likely different mutation spectrum and penetrance in this ethnic group.

Future directions

Despite our understanding of the BRCA1/BRCA2 mutations, there remain many unanswered questions. A large number of VUS still need to be classified as pathogenic or not, and due to their low frequencies of occurrence, some variants will probably never be classified. There are also increased reports of BRCA1/BRCA2 missense mutations, particularly in the less tested ethnicities, some of which have already been classified as pathogenic. Such reports are likely to increase when more genetic tests are being performed in different ethnic groups. Characterising VUS in familial breast cancers will be the future direction of the ABRCA Consortium group and will be included in the separated study.

Functional studies of BRCA1 and BRCA2 can provide valuable information on their roles in cancer development. Regardless of entering the era of next-generation high-throughput sequencing, many mutations in BRCA1 and BRCA2 to date still remain unclassified in terms of their pathogenicity, and much work would need to be done to better understand the mutations of these genes, particularly in different ethnic populations. The establishment of an Asian registry of BRCA1/BRCA2 mutation carriers would allow more organised research work to be done on this population.

Conclusion

BRCA1/BRCA2 mutations have been identified to be the main contributor of hereditary breast cancer, which increases the lifetime risk of breast cancer in women. The overall prevalence of BRCA1/BRCA2 mutations in Asians is comparable to that in other ethnic groups. In most Asian countries, the frequency of mutations in BRCA1 is similar or slightly higher than that in BRCA2, except in Korea and Philippines. With the knowledge of the mutation spectrum BRCA1/BRCA2 mutation carriers, improved genetic counselling and cancer management are likely to benefit Asian patients with breast cancer.

Acknowledgments

The authors would like to thank members of the Asian Hereditary Breast Cancer Consortium (ABRCA) for their contributions and support in this study, especially Drs Yuntao Xie, Zhi-Ming Shao, Guo-Jun Zhang, Ute Hamann, Samuel Haryono, Sue K. Park, Min Hyuk Lee, Jong Won Lee, Muhammad Usman Rashid, Rodney Dofitas, Peter Ang, Philip Iau, and Pimpicha Patmasiriwat. City of Hope Clinical Cancer Genetics Community Research Network and the Hereditary Cancer Research Registry, supported in part by Award Number RC4CA153828 (PI: J. Weitzel) from the National Cancer Institute and the Office of the Director, National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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Footnotes

  • Correction notice This article has been corrected since it published Online First. The acknowledgements section has been updated.

  • Contributors AK, EK, SN, SHT, ASGL, J-HS, OMG, JNW, SAN, JMF and S-WK conceived and designed the study. AK, JCWH, EK, SN, S-HT, ASGL, J-HS, OMG, AWK, JNW, M-TS, FBFL, T-LC, ESKM and SWK contributed to data acquisition. VYS and JCWH interpreted data and drafted the manuscript. AK, VYS, JCWH, SN, SHT, OMG, AWK, T-LC, SAN, JMF, ESKM and S-WK reviewed the manuscript. All authors approved the final manuscript.

  • Funding This study was partly supported by the Dr Ellen Li Charitable Foundation, the Kerry Group Kuok Foundation Limited, the National Institutes of Health (1R03CA130065), the National R&D Program for Cancer Control, Ministry for Health, Welfare, and Family Affairs, Republic of Korea (#1020350), the Canadian Breast Cancer Research Alliance and the Canadian Cancer Society Research Institute.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval Institutional Review Board of the University of Hong Kong.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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