Association of haplotypes spanning PDZ-GEF2, LOC728637 and ACSL6 with schizophrenia in Han Chinese
- 1State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
- 2Kunming Primate Research Centre, Chinese Academy of Sciences, Kunming, China
- 3Yunnan Mental Health Hospital, Kunming, China
- 4Graduate School of Chinese Academy Sciences, Beijing, China
- 5Virginia Institute for Psychiatric and Behavioral Genetics and Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, USA
- Professor B Su, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China;
- Received 25 May 2008
- Revised 1 July 2008
- Accepted 10 July 2008
- Published Online First 21 August 2008
Background: Schizophrenia is a complex genetic disorder caused by multiple genetic and environmental factors. Several lines of linkage and association studies have repeatedly suggested that the chromosome 5q22-33 region is implicated in the aetiology of schizophrenia. However, most of the previous studies on the linkage of 5q22–33 with schizophrenia were from European populations, and it was not well characterised in other populations.
Methods: We analysed eight single nucleotide polymorphisms (SNPs) located in the 5q23.3 region in a cohort of 506 schizophrenia patients and 672 control subjects from south western China. Single marker association, haplotypic association, sex-specific association and molecular evolutionary analysis were performed.
Results: Single marker analysis indicated that SNP5 (rs1355095) in LOC728637 is associated with schizophrenia. When males and females were analysed separately, SNP4 (rs31251) in PDZ-GEF2 is associated with schizophrenia in females. Further analysis using haplotypes demonstrated that a haplotype block spanning PDZ-GEF2, LOC728637 and ACSL6 is highly associated with schizophrenia and several haplotypes in this haploblock have about twofold to 10-fold increase in the affected subjects. In addition, molecular evolutionary analysis suggests that PDZ-GEF2 has undergone adaptive evolution due to Darwinian positive selection in the human lineage.
Conclusion: Our data provide evidence of the association of 5q22–33 with schizophrenia in Han Chinese. This chromosomal region is likely responsible for genetic susceptibility to schizophrenia, supporting previous data from European patients. In addition, our evolutionary analysis is consistent with the hypothesis that genes contributing to schizophrenia are likely under positive selection during human evolution.
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Funding: This study was supported by grants from the National 973 project of China (2006CB701506,2007CB815705), the Chinese Academy of Sciences (KSCX1-YW-R-34), the National Natural Science Foundation of China (30370755, 30525028 and 30630013), and the Natural Science Foundation of Yunnan Province of China.
Competing interests: None.
Patient consent: Obtained.