Article Text

PDF
Significant association of a M129V independent polymorphism in the 5′ UTR of the PRNP gene with sporadic Creutzfeldt-Jakob disease in a large German case-control study
  1. C Vollmert1,
  2. O Windl2,3,
  3. W Xiang2,
  4. A Rosenberger4,
  5. I Zerr5,
  6. H-E Wichmann6,
  7. H Bickeböller4,
  8. T Illig1,
  9. the KORA group,
  10. H A Kretzschmar2
  1. 1Institute of Epidemiology, GSF-National Research Center for Environment and Health, Neuherberg, Germany
  2. 2Center for Neuropathology and Prion Research (ZNP), Ludwig-Maximilians-University, Munich, Germany
  3. 3Veterinary Laboratories Agency, Weybridge, UK
  4. 4Department of Genetic Epidemiology (GEM), University of Göttingen, Göttingen, Germany
  5. 5Department of Neurology, Georg-August University, Göttingen, Germany
  6. 6Institute of Medical Informatics, Biometry and Epidemiology, Chair of Epidemiology, Ludwig Maximilians University, Munich, Germany
  1. Correspondence to:
 Professir Dr med H A Kretzschmar
 Center for Neuropathology and Prion Research, Ludwig Maximilians University Munich, Feodor-Lynen-Straße 23, D-81377 Munich, Germany; hans.kretzschmar{at}med.uni-muenchen.de

Abstract

Background: A single nucleotide polymorphism (SNP) in the coding region of the prion protein gene (PRNP) at codon 129 has been repeatedly shown to be an associated factor to sporadic Creutzfeldt-Jakob disease (sCJD), but additional major predisposing DNA variants for sCJD are still unknown. Several previous studies focused on the characterisation of polymorphisms in PRNP and the prion-like doppel gene (PRND), generating contradictory results on relatively small sample sets. Thus, extensive studies are required for validation of the polymorphisms in PRNP and PRND.

Methods: We evaluated a set of nine SNPs of PRNP and one SNP of PRND in 593 German sCJD patients and 748 German healthy controls. Genotyping was performed using MALDI-TOF mass spectrometry.

Results: In addition to PRNP 129, we detected a significant association between sCJD and allele frequencies of six further PRNP SNPs. No significant association of PRND T174M with sCJD was shown. We observed strong linkage disequilibrium within eight adjacent PRNP SNPs, including PRNP 129. However, the association of sCJD with PRNP 1368 and PRNP 34296 appeared to be independent on the genotype of PRNP 129. We additionally identified the most common haplotypes of PRNP to be over-represented or under-represented in our cohort of patients with sCJD.

Conclusion: Our study evaluated previous findings of the association of SNPs in the PRNP and PRND genes in the largest cohorts for association study in sCJD to date, and extends previous findings by defining for the first time the haplotypes associated with sCJD in a large population of the German CJD surveillance study.

  • BSE, bovine spongiform encephalopathy
  • KORA S4, Kooperative Gesundheitsforschung im Raum Augsburg, survey 4
  • LD, linkage disequilibrium
  • MALDI-TOF, matrix assisted laser desorption ionisation-time of flight
  • MS, mass spectrometry
  • PRND, prion-like doppel gene
  • PRNP, prion protein gene
  • SAP, shrimp alkaline phosphatase
  • sCJD, sporadic Creutzfeldt-Jakob disease
  • SNP, single nucleotide polymorphism
  • SSCP, single strand conformational polymorphism
  • prion protein gene
  • prion-like doppel gene
  • Creutzfeldt-Jakob disease
  • single-nucleotide polymorphism
  • genetic association study

Statistics from Altmetric.com

Footnotes

  • The KORA group consists of H-E Wichmann (speaker), H Löwel, C Meisinger, T Illig, R Holle, J John, and their coworkers, who are responsible for the design and conduct of the KORA studies.

  • The first two authors contributed equally to this work.

  • Competing interests: there are no competing interests.

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.