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J Med Genet 2004;41:867-871 doi:10.1136/jmg.2004.020941
  • Letters to JMG

The ser9gly SNP in the dopamine D3 receptor causes a shift from cAMP related to PGE2 related signal transduction mechanisms in transfected CHO cells

  1. M Hellstrand1,
  2. E A Danielsen2,
  3. V M Steen2,
  4. A Ekman1,
  5. E Eriksson1,
  6. C L Nilsson1
  1. 1Department of Pharmacology, Institute of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden
  2. 2Dr E Martens’ Research Group for Biological Psychiatry, Centre for Medical Genetics and Molecular Medicine and Locus on Neuroscience, Haukeland University Hospital, University of Bergen, Bergen, Norway
  1. Correspondence to:
 Monika Hellstrand
 PO Box 431, Göteborg SE 405 30, Sweden; monika.hellstrandpharm.gu.se

    The dopamine D3 receptor is a member of the D2 family of dopamine receptors. Several investigators have suggested that D3 receptors are involved in the regulation of locomotion,1 in the pathophysiology of schizophrenia,2 and in drug abuse.3

    Dopamine enhances basal and stimulus evoked release of arachidonic acid, as well as the production of one of its metabolites, prostaglandin E2 (PGE2), in CHO (Chinese hamster ovary) cells transfected with the D2 receptor.4–6 In contrast, dopamine does not influence basal arachidonic acid release7–9 but inhibits stimulus evoked release in cells transfected with the rat D3 receptor.10 On the other hand, with respect to cAMP production, the effect of dopamine is similar in CHO cells transfected with either D2 or D3 receptors, both receptor subtypes mediating a reduction in forskolin induced cAMP formation.11,12

    A single nucleotide polymorphism (SNP) in the first exon of the dopamine D3 receptor gene, the ser9gly polymorphism, leads to a serine to glycine amino acid substitution in the N-terminal extracellular domain of the receptor protein.13 An association between this polymorphism and schizophrenia has been suggested, but also questioned,14,15 a meta-analysis suggesting the effect to be small but real in white subjects.16 Other investigators have suggested that the gly-9 allele may be associated with an increased risk of developing antipsychotic induced tardive dyskinesia rather than with schizophrenia per se17; this finding also gained support from a report containing both pooled analyses of original data and a meta-analysis,18 and also from a recent primate study19 (see also a recent report by Lohmueller and coworkers20). In addition, the ser9gly polymorphism has been reported to be associated with therapeutic response to atypical antipsychotic agents, …

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