Expression of Gja1 correlates with the phenotype observed in oculodentodigital syndrome/type III syndactyly
- 1School of Biological Sciences and Department of Dental Medicine and Surgery, University of Manchester, Manchester, M13 9PT, UK
- 2Academic Unit of Medical Genetics and Regional Genetic Service, St Mary’s Hospital, Manchester, M13 0JH, UK
- 3Department of Paediatric Ophthalmology, University of Gent, De Pintelaan 185, B-9000 Ghent, Belgium
- Correspondence to: Professor M J Dixon School of Biological Sciences and Department of Dental Medicine and Surgery, 3.239 Stopford Building, University of Manchester, Oxford Road, Manchester, M13 9PT, UK;
- Accepted 31 July 2003
- Revised 3 July 2003
Oculodentodigital syndrome (ODD; OMIM 164200) is a congenital disorder characterised by developmental abnormalities of the face, eyes, limbs, and dentition. ODD is inherited in an autosomal dominant fashion and displays high penetrance but variable expression.1 In addition, a high rate of de novo mutations is observed.2 Facially, affected patients exhibit a long, narrow nose with hypoplastic alae, thin, anteverted nostrils and a prominent nasal bridge, short palpebral fissures, and bilateral microcornea often with anomalies of the iris.3,4 Secondary glaucoma occurs in a number of patients.5 Bilateral complete syndactyly of the fourth and fifth fingers (type III syndactyly) is the characteristic digital malformation. The third finger may occasionally also be involved and associated camptodactyly is a common finding.2 In addition, microdontia and generalised hypoplasia of the enamel, which tends to affect both the primary and secondary dentitions, are frequently observed.2,6 Cleft palate has also been reported in a number of cases.6–9 Less common features include thin, sparse hair and conductive deafness. Spastic paraparesis or lower limb weakness in association with ODD has been reported in a number of sporadic and familial cases.2,6,9–11 In two of these reports, magnetic resonance imaging demonstrated an underlying leukodystrophy and it has, therefore, been proposed that the definition of ODD be widened to include these features.10,11 Type III syndactyly has also been reported to occur as an isolated entity in several autosomal dominant pedigrees and it is uncertain whether this anomaly and ODD are separate genetic entities or part of the same disease spectrum.12–15 However, a family has been reported who, while not exhibiting the usual ocular or dental anomalies associated with ODD, did have a facial appearance that appeared to bridge the gap between the two conditions.16
The ODD locus was initially mapped to a 28 cM region of human chromosome 6q22-q24,17 which was subsequently refined to the 1.9 cM genetic interval delineated by the polymorphic markers D6S261, proximally, and D6S1639, distally.18 This study further indicated that ODD with associated neurological defects maps to this same interval. Recently, Paznekas and coworkers reported that the pleiotropic phenotype of ODD arises as the result of mutation of GJA1, the gene encoding the gap junction protein, connexin 43.19 In the current paper, we report nine different missense mutations, seven of which have not been described previously, in 10 unrelated families and confirm that type III syndactyly, at least in a subset of cases, is allelic with ODD. Using whole mount in situ hybridisation to analyse a developmental series of morphologically-staged embryos, we further demonstrate that there is a strong correlation between the sites of Gja1 expression and the clinical phenotype.
PATIENTS, MATERIALS AND METHODS
The pedigrees of six of the families analysed in the current study have been presented previously.17 The remaining five families were referred by clinical geneticists and ophthalmologists after a diagnosis of ODD had been made.
Oculodentodigital syndrome (ODD) is an autosomal dominant disorder characterised by developmental abnormalities of the face, eyes, limbs, and dentition. Mutations in GJA1, which encodes the gap junction protein connexin 43, have been shown to underlie ODD.
Here, we describe an additional 10 mutations in GJA1, seven of which are novel, bringing the total reported to date to 24. All but one of these mutations result in the introduction of a missense change into the amino terminal two thirds of connexin 43 highlighting the functional importance of this region of the protein. One of these mutations occurs in a family that exhibits type III syndactyly but not the usual ophthalmic, skeletal, or dental findings commonly associated with ODD.
Analysis of a developmental series of morphologically staged mouse embryos using whole mount in situ hybridisation allowed us to demonstrate a strong correlation between the spatio-temporal expression pattern of Gja1 in the developing craniofacial complex and limbs and the pleiotropic features of ODD.
GJA1 contains two exons, the coding sequence being encompassed in its entirety by the second exon (GenBank accession numbers: cDNA, M65188; genomic DNA, AL139098). The coding sequence of GJA1 was amplified in two overlapping segments using the primers 5′-AAT ACG TGA AAC CGT TGG TAG-3′ and 5′-CTC TTT CCC TTA ACC CGA TC-3′, which amplified a product of 855 bp, and 5′-TCT TTG AGG TGG CCT TCT TG-3′ and 5′-TAA GGC TGT TGA GTA CCA CC-3′, which amplified a product of 773 bp. The PCR amplifications were performed in 25 μl volumes containing 50 pmol of each primer, 200 μM dNTPs, 10 mM Tris-HCl pH 8.3, 50 mM KCl, 1.0 mM MgCl2 and 0.01% gelatin. The samples were overlaid with mineral oil, heated to 96°C for 10 min and cooled to 55°C. After addition of 0.75 U Taq DNA polymerase, the samples were processed through 35 amplification cycles of 92°C for 60 s, 55°C for 60 s, and 72°C for 60 s using a Hybaid thermal cycler. The final extension step was lengthened to 10 min. Negative controls were established for all reactions. After amplification, the products were excised from a 1% agarose gel and sequenced directly using the dideoxy chain termination method and dye primer chemistry. Primers were designed to avoid amplification of the processed pseudogene GJA1P1 on human chromosome 5q21-q22.
Whole mount in situ hybridisation
Sense and anti-sense riboprobes were generated from a Gja1 cDNA clone (IMAGE clone 1228506) that encompassed the last 390 bp of coding sequence plus 1726 bp of the 3′ untranslated region using the T3 and T7 promoters, respectively. Embryos dissected from time mated MF1 mice were fixed in 4% paraformaldehyde overnight, processed and subjected to hybridisation with sense and anti-sense probes as described previously20 with the modifications that hybridisations were performed at 60°C for 3–4 days and the detection reaction was performed at 4°C.
Analysis of the entire GJA1 coding sequence and the associated splice junctions resulted in the identification of nine different mutations, seven of which have not been reported previously, in the affected members of 10 out of the 11 ODD families (fig 1, table 1). No mutations were found in DNA samples from the two affected individuals from the remaining family. In each case the mutation segregated with the disease phenotype and was not present in 100 normal chromosomes. Each mutation resulted in the introduction of a missense change into connexin 43 and all of the mutated amino acid residues are highly conserved across connexin 43 orthologues (fig 2). The mutations are spread throughout the protein with three falling in the first transmembrane domain (nt79T>C resulting in S27P; nt93T>G resulting in I31M; nt119C>T resulting in A40V), one in the first extracellular loop (nt206C>A resulting in S69Y), four in the cytoplasmic loop (nt338T>C resulting in L113P; nt402G>T resulting in K134N; nt427G>A resulting in G143S; nt443G>A resulting in R148Q), and one in the second extracellular loop (nt605G>A resulting in R202H) (fig 2). The mutation G143S is of particular interest as it underlies the atypical phenotype of the family reported by Brueton and coworkers, members of which display type III syndactyly but none of the usual ophthalmological, dental or skeletal features commonly reported in ODD.16 The mutations A40V and R202H have been reported previously.19 A mutation of the lysine residue at position 134 has also been reported previously,19 however, the mutation identified in the current study is different as it results in a change to asparagine rather than glutamic acid. Interestingly, two of the mutations that we have identified (nt119C>T resulting in A40V and nt443G>A resulting in R148Q) result in a change to a nucleotide that is present in the GJA1 pseudogene (GJA1P1) located on human chromosome 5q21-q22, which shares 97% identity with GJA1. However, we can exclude the possibility that we have inadvertently amplified GJA1P1 as none of the other nucleotides that define the GJA1P1 sequence were detected in our amplification products. Interestingly, one of these mutations, R148Q, was detected in two unrelated families.
To correlate the expression of Gja1 during embryogenesis with the phenotypic features of ODD, a developmental series of morphologically staged mouse embryos was analysed using whole mount in situ hybridisation.
Expression in the craniofacial complex
Strong expression of Gja1 was detected throughout the craniofacial region at E8.5; this was particularly marked in regions anterior to the pre-otic sulcus at the rhombomere 2/3 boundary, in the most anterior portion of the forebrain surrounding the optic pits, and in the developing neural folds (figs 3A, 3B). Strong expression was also observed in the caudal neuropore. At E9.5, expression of Gja1 was detected in the frontonasal process and the developing branchial arches. In particular, the maxillary and mandibular portions of the first branchial arch and the second branchial arch exhibited strong expression (figs 3C, 3D). At E10.5, a gradient in the expression domain of Gja1 was evident in the developing mandible with the highest levels concentrated towards the midline (fig 3F). In addition, domains of Gja1 expression, very similar to those of Bmp4,21 were detected in the medial and lateral nasal processes, at the most proximal regions surrounding the nasal pit (fig 3E). Gja1 expression was also detected in the developing eye. At E12.5, expression was detected in the developing snout, particularly in the hair follicles and the primordia of the vibrissae. At E13.5, Gja1 expression was detected in the incisor and molar tooth germs. Although Gja1 expression was not detected in the palatal shelves prior to their elevation (E13), strong expression was detected as they underwent fusion at E14.5 (figs 3G, 3H). Gja1 expression was not, however, detected after completion of palatal fusion on E15 (data not shown).
Expression of Gja1 in the developing limb
At E9.5, strong expression of Gja1 was detected in the limb bud and the somites (fig 4A). At E10.5, a broad expression domain was present in the limb bud, however, by E11.5, this domain became localised predominantly to the apical ectodermal ridge (figs 4B, 4C). No obvious gradients in the expression of Gja1 along the anterior-posterior axis of the limb bud were observed. From E12.5, the expression within the apical ectodermal ridge persisted but areas of reduced expression were apparent in the interdigital regions (fig 4D). At E13.5, Gja1 expression was localised to the surface ectoderm of the limbs, in regions corresponding to the future digits (fig 4E). Strong expression domains were observed at the most distal portions of the digits. At E14.5 and E15.5, Gja1 expression was present in the dorsal and ventral surfaces of the digits and the hair follicles in the wrist (figs 4F, 4G).
This wide ranging expression pattern of Gja1 correlates with the pleiotropic phenotype observed in patients with ODD as the main areas of strong expression are in the eye, limbs, dentition, nasal region, fusing secondary palate, and hair follicles.
Connexin 43, encoded by GJA1, belongs to a family of transmembrane proteins each of which is composed of intracytoplasmic amino and carboxy termini and four membrane spanning domains linked by two extracellular and one intracellular loop. Six connexin proteins form a hemichannel or connexon which spans the cell membrane allowing connexons from neighbouring cells to dock to form a complete channel or gap junction. Gap junctions allow the exchange of secondary messengers, ions, and other small molecules up to the size of 1 kDa between adjacent cells thereby forming a system of cell-cell communication that operates alongside ligand-receptor signalling. Gap junction communication plays a crucial role not only in normal tissue physiology but in the regulation of information flow required during embryonic morphogenesis.22 Mutations in the genes encoding various connexins have been demonstrated to underlie a wide range of genetic disorders including Charcot-Marie-Tooth syndrome, deafness, dermatological disorders, cataracts and heart malformations, suggesting a wide range of expression patterns and functional diversity.23–25
Recently, Paznekas and coworkers showed that mutations in GJA1 underlie the congenital malformation oculodentodigital syndrome.19 In the current investigation, we have extended these findings by delineating a further nine GJA1 mutations, seven of which are novel, in 10 ODD families bringing the total number of different mutations reported to date to 24. One of these mutations was found to underlie the atypical phenotype of the family reported by Brueton and coworkers16 and our observations therefore indicate that type III syndactyly, at least in this family, is allelic with typical ODD. All but one of the mutations that have been reported to date lead to missense changes in connexin 43, the remaining mutation being a duplication of a phenylalanine residue at position 52. In all cases, the mutations occur in amino acid residues that are highly conserved during evolution (fig 2). The lack of mutations resulting in the introduction of a termination codon into the protein suggests that the mechanism underlying ODD is not a loss of connexin 43 function. This hypothesis is reinforced by the observation that nullizygosity of Gja1 in mice does not lead to an ODD-like phenotype. Rather, mice lacking connexin 43 died at birth, as a result of a failure in pulmonary gas exchange caused by swelling and blockage of the right ventricular outflow tract from the heart.26 These observations suggest that a dominant negative or “gain of function” mechanism might underlie ODD.
The 24 different mutations reported to underlie ODD are distributed throughout the different functional domains of the first two thirds of connexin 43 (fig 2). Most of the 5′ mutations that have been described fall within the 19 amino acid N-terminal domain of connexin 43 (Y17S, S18P,19), a region that has been shown to be involved in voltage gating of gap junction channels in connexins 26 and 32.27,28 Eight of the mutations that have been described to date (G21R, G22E, K23T, S27P, I31M, A40V, L90V, V216L) occur in the transmembrane domains of connexin 43, the integrity of which is known to be essential for correct transport of the protein into the plasma membrane.29 While the precise contribution of each domain to the formation of the pore of the gap junction, and therefore channel permeability, remains controversial, the first transmembrane domain has been strongly implicated in this process.30,31 It is therefore interesting that six of the transmembrane mutations identified in ODD families fall within this region of connexin 43. In total, five mutations (Q49K, F52dup, S69Y, R76S, R202H) have now been identified in the two extracellular loops of connexin 43. Mutations in this region of connexin 43 might interfere with relative spacing of the cysteine residues in the extracellular loops, which is critical for appropriate disulphide bond formation and the specific docking of two hemichannel connexons to form a functional gap junction.32 In this context, Paznekas and coworkers19 reported the mutation Q49K in one ODD family. Mutation of the glutamine residue at position 49 to histidine in chick connexin 43 did not allow the production of connexin 43 channels, most probably through the distortion of the secondary structure of the first extracellular loop.33 Alternatively, the mutations may interfere with the voltage gating of the entire channel. The remaining nine mutations that have been identified in GJA1 affect the cytoplasmic loop of connexin 43. The amino acid composition and size of this region of the connexin family is highly diverse, nevertheless, all of the mutations that have been identified in this region of connexin 43 affect highly conserved residues (fig 2). Moreover, antibodies directed against amino acids 123–136 and 131–142 of connexin 43, which encompass I130, K134 and G138 that are mutated in ODD, prevented intercellular communication through mouse embryo gap junctions.34 In summary, the consequence of any of the 24 mutations found in connexin 43 to date may be to disrupt the docking of the connexins to form a hemichannel or the two hemichannels to form an entire gap junction, or to affect the permeability of the fully formed gap junction. Interestingly, no ODD mutations have, as yet, been identified in the 149 amino acid carboxy terminal cytoplasmic domain. This distribution suggests that mutations in this region of connexin 43 might lead to a different phenotype. In this context, the S365P mutation is thought to result in autosomal recessive cardiac malformations with laterality defects in a subset of cases.35
The strong correlation between the sites of Gja1 expression during mouse embryonic development and the ODD phenotype provide further support for mutations in GJA1 underlying ODD. The earliest stages examined in the current study (E8) allowed us to demonstrate that Gja1 is expressed in the most anterior portion of the forebrain and in the developing neural folds. These results confirm the earlier report of Lo and coworkers36 and are consistent with the proposed role of connexin 43 in the modulation of neural crest cell motility.37 Similarly, the Gja1 expression patterns that we observed in the branchial arches and developing facial processes mirror those reported for connexin 43 in the chick.38 The highest levels of chicken connexin 43 expression were concentrated in the mesenchyme at the distal edges of the medial nasal, lateral nasal and maxillary processes suggesting a role in fusion of the facial primordia.38 In this context, application of antisense oligonucleotides that specifically reduced the levels of connexin 43 protein in cells of the early chick facial primordia resulted in craniofacial anomalies consistent with those observed in ODD.39,40 Our observation that Gja1 is also specifically expressed in the medial edges of the paired palatal shelves immediately before and during fusion of the murine secondary palate is interesting because of the cleft palate that has been reported in a number of patients with ODD. This expression pattern implies a key role for connexin 43 in the adhesion or fusion of the palatal shelves. The process of epithelial-mesenchymal transformation has been implicated in palatal shelf fusion41,42 and, in this context, it is perhaps significant that gap junction communication is an important regulator of epithelial-mesenchyma transformation during heart development.43 Additional craniofacial expression of Gja1 was detected in the developing eyes, vibrissae, and molar and incisor tooth germs mirroring the ocular anomalies, abnormal hair growth, and microdontia/enamel hypoplasia observed in patients with ODD. These results are also consistent with the reported expression of connexin 43 in rat incisor odontoblasts,44,45 human dental pulp fibroblasts46 and during ocular development in Xenopus, mouse and chick.47–49
Finally, we have shown that Gja1 is expressed across the entire limb bud at E9, but, by E10, Gja1 is restricted to the AER. Meyer and coworkers have previously shown that Gja1 is dynamically expressed during early limb development in the mouse and that ectopic expression of Wnt1 in the limb mesenchyme leads to alterations in Gja1 expression in conjunction with limb abnormalities.50 Similarly, Gja1 is expressed during limb development in Xenopus and chick39,48 and knockdown of Gja1 in chicks leads to limb anomalies.39 Later in limb development, expression of Gja1 is progressively down-regulated in the interdigital webs but retained in the digits themselves. As with the craniofacial expression of Gja1, these results are consistent with the syndactyly observed in ODD.
Taken together, our results indicate that connexin 43 plays a key role in normal facial and limb development and that mutation of GJA1 results in craniofacial anomalies and limb abnormalities. Nevertheless, the precise way in which mutant connexin 43 disrupts gap junction communication and leads to the malformations observed in ODD remains to be elucidated.
We thank the families for participating in the study and A Aylsworth, L Brueton, S Farrell, A Fryer, R Hennekam, K Metcalfe, A Murray, C Schrander-Stumpel, H Toriello, A Verloes, and R Winter for providing samples.
We should also like to thank E De Baere and L Messiaen for critical appraisal of the manuscript.
This work was supported by grants from the Wellcome Trust (058423, 069243).