Table 1
Primer sequences used for LD-PCR and breakpoint specific PCR
| Case No | Name | Sequence | Direction |
|---|---|---|---|
| Long PCR primers | |||
| A | L4-6F1 | 5`-CCTTTGGTGAGGTGACAGTGGGTGA-3` | Sense |
| L4-6R1 | 5`-TGTCCTGGCAAAAGCGAGGTCTTAA-3` | Antisense | |
| B | L10-3F1 | 5`-CCTCAACCAAGACTCACAAGGAACA-3` | Sense |
| L10-3R1 | 5`-TGGCTAAATCCTCAAAGGACTGCA-3` | Antisense | |
| C | Ex11F | 5`-TCCCACTATCTAAGGTAATTG-3` | Sense |
| Ex14R | 5`-GGACCATTGTTGTAGTAGCTC-3` | Antisense | |
| PCR primers spanning the mutation breakpoint | |||
| A | L1 Int 4-5 F2 | 5`-AATCCAATTCAAGGACTGGG-3` | Sense |
| L1 Int 4-5 R2 | 5`-AACTCACCACAACCTCTGCC-3` | Antisense | |
| B | L1 Int 10-2F4 | 5`-CAGGAGGTGCTGATAGCTTG-3` | Sense |
| L1 Int 10-2R3 | 5`-TGAGCATCTTTTCAGGTGAG-3` | Antisense | |
| C | ML1 11-F6 | 5`-CCAGCAGGAGGAGCATAATG-3` | Sense |
| ML1 13-R10 | 5`-GGATGTCAACCAGATACCAGTG-3` | Antisense |
