Table 2
List of primers
| Primer name | Sequence | Derived from GenBank accession number |
|---|---|---|
| To allow the discrimination between alleles D114 and 114Y (340G→T) the primers pCANmF and pCANmR were modified in the following way. For pCANmF the nine nucleotides at the 5` end are derived from the 3` splice acceptor of intron 1.4 In order to create an internal SnaBI control restriction site nucleotides C21, A22, and A24 (in bold, numbered from the 5` end) were replaced with A, C, and T (underlined), respectively. For pCANmR, nucleotides A25 and G27 were replaced with T and C (underlined), respectively. These replacements allowed SnaBI digestion of the mutant 114Y allele while leaving the D114 allele resistant. | ||
| cDNA primers | ||
| CAF1 | 5`CTGTACTTTGCCCTTTTGGTA | S67156.1 |
| CAF3 | 5`CTGATTGAGCATCCTTCCCTCA | S67156.1 |
| CAR3 | 5`ACACCGTGTAAGATGTAAGCT | S67156.1 |
| Genomic DNA primers | ||
| CAex1F | 5`CTTCTGAATTGCAGAAATCAGA | I70466 |
| CAex1R | 5`CACACCTACCACTTTCACACAA | |
| CAex2F | 5`CTCAGGCACAGATGTTGTTCA | I70467 |
| CAex2R | 5`CAAGTCCTTTGCTGACTTATAA | |
| CAex3F | 5`GGGTTTTTACCCAAGAAAGACGT | I70468 |
| CAex3R | 5`CTCTGAGTTTCAGCTAGGACAT | |
| CAex4F | 5`ATGTGACTATCTCCTTCTGT | I70469 |
| CAex4R | 5`TCTGACCCAGGTTCCAATTGTTA | |
| CAex5F | 5`CCAGAGATGTTTTTAGTTGCCA | I70470 |
| CAex5R | 5`TGCTGTATGAGCTATAAACTTCT | |
| CAex6F | 5`GAGTCTGACATAAATTTTTAGAGGA | I70471 |
| pCANmF | 5`TTATAACAGCAAAAAAATGTACGTAGATTTGCCATATGAAGTG | |
| pCANmR | 5`CCCCATGTTAGAGGTGGTGTTGTGTACGT | |
