Table 1
Primers used for DGGE analysis of the L1CAM gene. Shown are the primer sequences, the amplicon size, and the pools in which the DGGE products can be analysed
| Exon | Pool | Length (bp) | Primer | Primer sequence |
|---|---|---|---|---|
| 1 | II | 151 | LC-1F | GTGGCTGTGCTGCGCGGTGC |
| LC-1Rgc | (40GC)CATAGCGGCGAAGGTAGGCG | |||
| 2 | V | 107 | LC-2Fgc | (40GC)TCTCTCCATTCCCTCCCTTG |
| LC-2R | TACCCTTCTCCTTGGCCTTC | |||
| 3 | IV | 287 | LC-3Fgc | (40GC)GTGCTGAGGCTATGACACCA |
| LC-3R | GAAGTTAGGCAGTCCAGGGA | |||
| 4 | X | 376 | LC-4F1gc | (40GC)CACCTGTCCCTCCAGCCTGT |
| LC-4R | CACAATCCCACACGAACTCC | |||
| 5 | IV | 197 | LC-5Fgc | (55GC)GAGGAGAGTGTCAGCCCGTC |
| LC-5R | TAACACCCCGACCCCACGAG | |||
| 6 | IX | 279 | LC6F | GTGTCTTCCTGGACGGGGTC |
| LC-6Rgc | (40GC)AAGGGCCATGCCTGAGGGTG | |||
| 7 | V | 278 | LC-7Fgc | (55GC)AATTCTGGGGTGGAGGGAAG |
| LC-7R | TGGTCTGAGCTCCCTGCTAG | |||
| 8 | VIII | 303 | LC-8F | CCCTTCTGCTCTCTTCCCTT |
| LC-8Rgc | (40GC)CATGACAGTGGGCATCACAG | |||
| 9 | III | 349 | LC-9F | CCTGTGATGCCCACTGTCAT |
| LC-9Rgc | (40GC)TTCCTCACCCTCCTCTTCCT | |||
| 10 | IX | 316 | LC-10F | GAAGAGGAGGGTGAGGAAGG |
| LC-10Rgc | (40GC)AGTGGGTGCAGGGACAGACT | |||
| 11 | II | 311 | LC-11Fgc | (40GC)AGCAGGGAACCAAGATTTGC |
| LC-11R | ACACGACACTCACCACTACC | |||
| 12A | I | 287 | LC-12AF | GTAGTGGTGAGTGTCGTGTC |
| LC-12ARgc | (40GC)AGCCATGATGGTAACATTGT | |||
| 12B | VII | 195 | LC-12BF | AATGACACCGGACGCTACTT |
| LC-12BRg | (40GC)TTTTTTTTTTTTTGTCACTCTGTTGTCCTCCAG | |||
| 13 | VI | 281 | LC-13Fgc | (40GC)ACAACAGAGTGACTTCCCCA |
| LC-13R | TTCCAGCTTTTCCCACTCTG | |||
| 14 | I | 228 | LC-14Fgc | (40GC)GGGAGGGATTGGGAGGGGAG |
| LC-14R | CCCGGCCTTCTGGAGTGGAG | |||
| 15 | VII | 317 | LC-15F | TGGGCCCTTTCAAGCACCGA |
| LC-15Rgc | (40GC)TTCCCATGAGGAACCGTGAG | |||
| 16A | X | 232 | LC-16Afgc | (40GC)CCCAAAGCCACATGCTGATC |
| LC-16AR | ACCACAGTCTCAGAGACCGG | |||
| 16B | XI | 153 | LC-16BFgc | (GC)ACACCTTTAGGGTTACTGCC |
| LC-16BR | TCTAAGCCCTCCCTTCACAG | |||
| 17 | VI | 220 | LC-17Fgc | (40GC)TCCTCGTGGCTCTCCAAAAG |
| LC-17R | CCCAGGTCATGGCCTCATGT | |||
| 18 | V | 405 | LC-18F | GGGGGGGCCAAAGAATGCTGGTGTT |
| LC-18Rgc | (40GC)ATGCTGAGAGGTGTGGACAT | |||
| 19 | VIII | 309 | LC-19Fgc | (40GC)TTCTCTGTGTGTAGGGGCTT |
| LC-19R | GGGGGGCTCACCATCCTGTCGCTTTA | |||
| 20 | VIII | 416 | LC-20Fgc | (40GC)GAGAGGAGGGTCCCCATTAA |
| LC-20R | ACAGAACCGAGTGGCAGGTA | |||
| 21 | VI | 228 | LC-21Fgc | (55GC)TACCTGCCACTCGGTTCTGT |
| LC-21R | CTCCACCTCCCTTCCCTGCT | |||
| 22 | II | 273 | LC-22F | CACAGCCAACCCCTGTCTGT |
| LC-22Rgc | (40GC)TCCCTGTTGGCAGGTCATTC | |||
| 23 | IV | 296 | LC-23Fgc | (40GC)TAAACCTTAGACTTCCTGGCAGCT |
| LC-23R | TGAGTCGGAGTGCCAGGCAA | |||
| 24 | X | 331 | LC-24Fgc | (40GC)TAAATTGCCTGGCACTCCGACTCA |
| LC-24R | GGGCCTTCAGGGGACAGAAGGACAT | |||
| 25 | VII | 357 | LC-25F | GGGGCCAGGGTCCCAACTTTAAGAGC |
| LC-25Rgc | (40GC)ATCCAGGAGGCCTTGCAGAA | |||
| 26/27 | IX | 383 | LC-26/27Fgc | (40GC)GCTGTTGAGACAGAGTGCTG |
| LC-26/27R | AGGCGCACATTGTCTATAGG | |||
| 28 | III | 429 | LC-28F | AAACAAATGGAAGGCAGGCG |
| LC-28RGC | (40GC)AGACAGCAAGTTCTCCTCTG |
