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J Med Genet 1984;21:164-172 doi:10.1136/jmg.21.3.164
  • Research Article

Blot hybridisation analysis of genomic DNA.

  1. S Vandenplas,
  2. I Wiid,
  3. A Grobler-Rabie,
  4. K Brebner,
  5. M Ricketts,
  6. G Wållis,
  7. A Bester,
  8. C Boyd,
  9. C Måthew

      Abstract

      Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.

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