ORIGINAL ARTICLE

Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays

H Fiegler^1,*, S M Gribble^1,*, D C Burford¹, P Carr¹, E Prigmore¹, K M Porter¹, S Clegg¹, J A Crolla², N R Dennis², P Jacobs^2,*, N P Carter^1,*

¹ The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
² Wessex Regional Genetics Laboratory, Salisbury, UK

Correspondence to:
Correspondence to:
Dr N P Carter
npc{at}sanger.ac.uk

Objective: The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.

Methods: Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.

Results and Conclusions: By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.

Abbreviations: FISH, fluorescence in situ hybridisation; CGH, comparative genomic hybridisation; RCP, reverse chromosome painting

Keywords: chromosome rearrangement; DNA microarrays; reverse chromosome painting; chromosome breakpoint

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